Summary
In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringent response. Using an in vitro transcription assay with intact organelles from both ϱ+ and ϱ− strains, we show here that the mitochondrial stringent response includes not only transcription of the 21S and 16S rRNA genes, but also that of organellar genes specifying non-mitoribosome-related products. Stringent organellar transcriptional curtailment is identical when cells are starved for a required (marker) amino acid or when they are subjected to nutritional downshift, and the relative level of that transcriptional curtailment following either perturbation is the same in cells growing on fermentative (repressing) or purely respiratory carbon sources. These results confirm that the mechanism governing mitochondrial gene expression during a stringent response is specified outside the organelle, and they show that this transcriptional control mechanism is not immediately subject to glucose repression. In all strains examined, stringent organellar gene expression requires a mitochondrial promoter, suggesting that the regulatory mechanism which functions during the stringent response operates primarily at transcriptional initiation.
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References
Birky CW (1975) Gene 80:695–709
Biswas T, Getz G (1986a) J Biol Chem 261:3927–3930
Biswas T, Getz G (1986b) Proc Natl Acad Sci USA 83:270–274
Biswas T, Edwards JC, Rabinowitz M, Getz G (1985) Biochemistry 82:1954–1958
Bradford M (1976) Anal Biochem 72:248–254
Chandrasekaran K, Jayarman J (1978) FEBS Lett 87:52–54
Christianson T, Rabinowitz M (1983) J Biol Chem 258:14025–14033
Donovan DM, Pearson NJ (1986) Mol Cell Biol 6:2429–2435
Dennis PP (1977) J Bacteriol 129:580–588
Edwards JC, Levens D, Rabinowitz M (1982) Cell 31:337–346
Elion EA, Warner JR (1986) Mol Cell Biol 6:2089–2097
Fang M, Butow RA (1970) Biochem Biophys Res Commun 41:1579–1583
Gallant JA (1979) Annu Rev Genet 13:393–415
Gibbs JB, Marshall MS (1989) Microbiol Rev 53:171–185
Greenleaf AL, Kelly JL, Lehman IR (1986) Proc Natl Acad Sci USA 83:3391–3394
Groot GFP, van Harten-Loosbroeck N, van Ommen G-JB, Pijst HLA (1980) Nucleic Acids Res 9:6369–6377
Hahn S, Guarente L (1988) Science 240:317–321
Hudspeth MES, Shumard DS, Tatti KM, Grossman L (1980) Biochem Biophys Acta 610:221–228
Kelly JL, Greenleaf AL, Lehman IR (1987) J Biol Chem 261:10348–10351
Kim CH, Warner JR (1983) Mol Cell Biol 3:457–465
Lamb MR, Anziano PQ, Glaus KR, Hanson DK, Klapper HJ, Perlman PS, Mahler HR (1983) J Biol Chem 258:1991–1999
Lisowsky T (1990) Mol Gen Genet 220:186–190
Lisowsky T, Michealis G (1989) Mol Gen Genet 219:125–128
Locker J, Rabinowitz M (1981) Plasmid 6:302–314
Masters BS, Stohl LL, Clayton DA (1987) Cell 51:89–99
McEntee CM, Hudson AP (1989) Anal Biochem 176:303–306
McEntee CM, Cantwell R, Thomas LC, Hudson AP (1989) Biochem Biophys Res Commun 164:362–369
Mueller DM, Getz G (1986a) J Biol Chem 261:11756–11765
Mueller DM, Getz G (1986b) J Biol Chem 261:11816–11822
Oliver SG, McLaughlin CS (1977) Mol Gen Genet 154:145–153
Pao CC, Paietta J, Gallant JA (1977) Biochem Biophys Res Commun 74:314–322
Ray DB, Butow RA (1979a) Mol Gen Genet 173:227–238
Ray DB, Butow RA (1979b) Mol Gen Genet 173:239–247
Richter D, (1973) FEBS Lett 34:291–294
Schinkel AH, Groot Koerkamp MJA, Van der Horst GTL, Touw EPW, Osinga KA, Van der Bliek AM, Veeneman GH, Van Boom JH, Tabak HF (1986) EMBO J 5:1041–1047
Schinkel AH, Groot Koerkamp MJA, Touw EPW, Tabak HF (1987) J Biol Chem 262:12785–12791
Shulman RW, Sripati CE, Warner JR (1977) J Biol Chem 252:1344–1349
Sor F, Fukuhara H (1983) Nucleic Acids Res 11:339–348
Warner JR, Gorenstein C (1978) Nature 275:338–339
Wettstein-Edwards J, Ticho BS, Martin NC, Najarian D, Getz G (1986) J Biol Chem 261:2905–2911
Winkley CS, Keller MJ, Jaehning JA (1985) J Biol Chem 260:14214–14223
Zassenhaus HP, Martin NC, Butow RA (1984) J Biol Chem 259:6019–6027
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Communicated by R. J. Rothstein
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Cantwell, R., McEntee, C.M. & Hudson, A.P. Regulation of mitochondrial transcription during the stringent response in yeast. Curr Genet 21, 241–247 (1992). https://doi.org/10.1007/BF00336848
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DOI: https://doi.org/10.1007/BF00336848