Summary
The host-controlled EcoK-restriction of unmodified phage λ.O is alleviated in dam mutants of Escherichia coli by 100- to 300-fold. In addition, the EcoK modification activity is substantially decreased in dam - strains. We show that type I restriction (EcoB, EcoD and EcoK) is detectably alleviated in dam mutants. However, no relief of EcoRI restriction (Type II) occurs in dam - strains and only a slight effect of dam mutation on EcoP1 restriction (Type III) is observed. We interpret the alleviation of the type I restriction in dam - strains to be a consequence of induction of the function which interferes with type I restriction systems.
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Arber W, Humbelin H, Caspers P, Reif HJ, Iida S, Meyer J (1980) Spontaneous mutations in the E. coli prophage P1 and IS-mediated processes. Cold Spring Harbor Symp Quant Biol 43:1197–1208
Arraj JA, Marinus MG (1983) Phenotypic reversal in dam mutants of Escherichia coli K12 by recombinant plasmid containing the dam + gene. J Bacteriol 153:562–565
Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K12. Bacteriol Rev 36:525–557
Bale A, d'Alarcao M, Marinus MG (1979) Characterization of DNA adenine methylation mutants of Escherichia coli K12. Mutat Res 59:165–175
Belogurov AA, Yussifov TN, Kotova VU, Zavilgelsky GB (1985) The novel gene(s) ard of plasmid pKM101: alleviation of EcoK restriction. Mol Gen Genet 198:509–513
bertani G, Weigle JJ (1953) Host-controlled variation in bacterial viruses. J Bacteriol 65:113–121
Braun R, Wright A (1986) DNA methylation differentially enhances the expression of one of two Escherichia coli dnaA promoters. Mol Gen Genet 202:246–250
Day SR (1977) UV-induced alleviation of K-specific restriction of bacteriophage λ. J Virol 21:1249–1251
Doutriaux MP, Wagner R, Radman M (1986) Mismatch-stimulated killing. Proc Natl Acad Sci USA 83:2576–2578
Glickman BW, Radman M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc Natl Acad Sci USA 77:1063–1067
Glickman BW, van den Elsen P, Radman M (1978) Induced mutagenesis in dam - mutants of Escherichia coli, a role of 6-methyladenine residues in mutation avoidance. Mol Gen Genet 163:307–312
Kato T, Shinoura Y (1977) Isolation and characterization of mutants of Escherichia coli deficient in induction of mutation by ultraviolet light. Mol Gen Genet 156:121–131
Lacks S, Greenberg B (1977) Complementary specificity of restriction endonuclease of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol 114:153–168
Little JW, Mount DW (1982) The SOS regulatory system of Escherichia coli. Cell 29:11–22
Lu A-L, Clark S, Modrich P (1983) Methyl-directed repair of DNA base-pair mismatches in vitro. Proc Natl Acad Sci USA 80:4639–4643
Marinus MG (1984) Methylation of procariotic DNA. In: Razin A, Cedar H, Riggs A (eds) DNA methylation. Springer-Verlag, New York Heidelberg Berlin, pp 81–109
Marinus MG (1985) DNA methylation influences trpR promoter activity in Escherichia coli K12. Mol Gen Genet 200:185–186
Marinus MG, Konrad EB (1976) Hyper-recombination in dam mutants of Escherichia coli K12. Mol Gen Genet 149:273–277
Marinus MG, Morris NR (1974) Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12. J Mol Biol 85:309–322
Marinus MG, Morris NR (1975) Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res 28:15–16
Marinus MG, Carraway M, Frey AZ, Brown L, Arraj JA (1983) Insertion mutations in the dam gene of Escherichia coli K12. Mol Gen Genet 192:288–289
McGraw BR, Marinus MG (1980) Isolation and characterization of Dam + revertants, and supressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K12. Mol Gen Genet 178:309–315
Messer W, Bellekes U, Lother H (1985) Effect of dam methylation on activity of the E. coli replication origin. EMBO J 4:1327–1332
Miller J (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, New York
Mizusawa S, Court D, Gottesman S (1983) Transcription of the sulA gene and repression of lexA. J Mol Biol 171:337–343
Peterson KR, Wertman KF, Mount DW, Marinus MG (1985) Viability of Escherichia coli K12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon. Mol Gen Genet 201:14–19
Plasterk RH, Vollering M, Brinkman A, van de Putte P (1984) Analysis of the methylation-regulated Mu mon transcript. Cell 36:189–196
Pukkila PJ, Peterson J, Herman G, Modrich P, Meselson M (1983) Effect of high level of DNA adenine methylation on methyldirected mismatch repair in Escherichia coli. Genetics 104:571–582
Roberts D, Hoopes BC, McClure WR, Kleckner N (1985) IS10 transposition is regulated by DNA adenine methylation. Cell 43:117–130
Roulland-Dussoix D, Boyer HW (1969) The Escherichia coli B restriction endonuclease. Biochim Biophys Acta, 195:219–229
Sengstag C, Arber W (1983) IS2 insertion is a major cause of spontaneous mutagenesis of the bacteriophage P1: non-random distribution of target sites. EMBO J 2:67–71
Smith DW, Garland AM, Herman G, Enns RE, Baker TA, Zyskind J (1985) Importance of state of methylation of oriC sites in initiation of DNA replication in E. coli. EMBO J 4:1319–1326
Sternberg N (1985) Evidence that adenine methylation influences DNA-protein interactions in E. coli. J Bacteriol 164:490–493
Studier FW (1975) Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol 94:283–295
Toms B, Wackernagel W (1982) UV-induced alleviation of restriction in E. coli K12: Kinetics of induction, and specificity of this SOS function. Mol Gen Genet 186:111–117
Wagner R, Meselson M (1976) Repair tracks mismatched DNA heteroduplexes. Proc Natl Acad Sci USA 73:4135–4139
Walker GC (1984) Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev 48:60–93
Zabeau M, Fridman S, Van Montagu M, Schell J (1980) The ral gene of phage λ I. Identification of a non-essential gene that modulates restriction and modification in Escherichia coli. Mol Gen Genet 179:63–74
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Efimova, E.P., Delver, E.P. & Belogurov, A.A. Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli . Mol Gen Genet 214, 313–316 (1988). https://doi.org/10.1007/BF00337727
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DOI: https://doi.org/10.1007/BF00337727