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Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids

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Summary

The buoyant density and endonuclease restriction patterns of potato (Solanum tuberosum L.) and tomato (Lycopersicon esculentum) ptDNA were examined and compared with those of their somatic hybrids. The plastids from these plants, both of which belong to the family of Solanaceae, contain a single DNA species whose density of 1.697 gcm-3 and size of approximately 156 kbp are similar to those of ptDNA from other higher plants. The Sal I restriction patterns were indistinguishable; however, those obtained with Kpn I, Pst I, and Eco RI disclosed that each species possesses a unique ptDNA. These observations suggest a relatively recent divergence of both species. Of the twelve hybrid lines screened, eight contained exclusively potato ptDNa and four contained only tomato ptDNA at a 0.1–3% level of detection. Rearrangements of modifications of DNA were not detected. The plastid identities of three hybrid lines that had previously been analyzed by isoelectric focusing of RuBPcase subunits (Melchers et al. 1978) agreed with those determined by restriction endonuclease analysis.

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Abbreviations

ptDNA:

plastid DNA, chloroplast DNA

cDNA:

copy DNA

RuBPcase:

ribulose bisphosphate carboxylase/oxygenase

LSU:

large subunit of RuBPcase

kbp:

kilobase pairs

SDS:

sodium dodecyl sulfate

SSC:

standard saline citrate

IEF:

isoelectric focusing

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Authors and Affiliations

  1. Botanisches Institut der Universität, D-4000, Düsseldorf 1, Federal Republic of Germany

    Barbara Schiller & R. G. Herrmann

  2. Max-Planck-Institut für Biologie, D-7400, Tübingen, Federal Republic of Germany

    G. Melchers

Authors
  1. Barbara Schiller
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  2. R. G. Herrmann
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  3. G. Melchers
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Communicated by J. Schell

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Schiller, B., Herrmann, R.G. & Melchers, G. Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol Gen Genet 186, 453–459 (1982). https://doi.org/10.1007/BF00337947

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  • DOI: https://doi.org/10.1007/BF00337947

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