Abstract
Spleen cells from an SJL mouse immunized with 70'/3 cells, an established pre-B cell line, were fused with cells of the nonsecretor myeloma line NS.1. One established hybridoma cell line (clone K10.6) continuously secreted antibody that recognized a new antigenic specificity tentatively named Ly-m19. This newly found antigen is detectable on both T and B cells. Cytotoxicity assays reveal that 75 percent of the spleen and lymph-node cells, 35 percent of bone-marrow cells, and 15 percent of thymus cells reacted with antibody of clone K10.6. Strains expressing the specificity Ly-m19.1 are characterized by negative reactions and include the strains AKR, CE/J, RF/J, GR/A, SJL, P/J, BDP/J, and LG/J. All other strains so far tested are Ly-m19.2. This strain distribution pattern distinguishes Ly-m19 from any known murine lymphocyte alloantigen, but it parallels the Lyb-2c haplotype. Linkage test of a set of AKXL recombinant inbred strains revealed close linkage of Ly-m19 and Lyb-2 loci on mouse chromosome 4.
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Abbreviations
- LPS:
-
lipopolysaccharide
- B6:
-
C57BL/6
- Con-A:
-
concanavalin A
- MLC:
-
mixed-lymphocyte culture
References
Eckhardt, L. A. and Herzenberg, L. A.: Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression. Immunogenetics 11: 275–291, 1980
Feeney, A. J. and Hammerling, U.: Ala-1: A murine alloantigen of activated lymphocytes. I. Serological analysis of mitogen-stimulated T and B cells. Immunogenetics 3: 369–379, 1976
Gorer, P. A. and O'Gorman, P.: The cytotoxic activity of isoantibodies in mice. Transplant. Bull. 3: 142–143, 1956
Hammerling, G. J., Hammerling, U., and Flaherty, L.: Qat-4 and Qat-5, new murine T-cell antigens governed by the Tla region, and identified by monoclonal antibodies. J. Exp. Med. 150: 108–116 1979
Kessler, S., Ahmed, A., and Scher, L.: Identification and partial molecular and genetic characterization of an alloantigen expressed on murine B lymphocytes. In M. Cooper, D. Mosier, I. Scher, and E. Vitetta (eds.): B Lymphocytes in the Immune Response, p. 47, Elsevier/North,Holland, Amsterdam, 1979
Kimura, S., Tada, N., and Hammerling, U.: A new lymphocyte alloantigen (Ly-10) controlled by a gene linked to the Lyt-1 locus. Immunogenetics 10: 363–372, 1980a.
Kimura, S., Tada, N., Nakayama, E., and Hammerling, U.: Studies of the mouse Ly-6 alloantigen system. I. Serological characterization of mouse Ly-6 alloantigen by monoclonal antibodies. Immunogenetics 11: 373–381, 1980b
Kimura, S., Tada, N., Liu, Y., and Hammerling, U.: A new mouse cell surface antigen (Ly-m18) defined by monoclonal antibody. Immunogenetics, in press, 1981a
Kimura, S., Tada, N., Nakayama, E., Liu, Y., and Hammerling, U.: A new cell surface antigen (Ly-m20) controlled by a gene linked to Mls locus and defined by monoclonal antibodies. Immunogenetics, in press, 1981b
Klein, J., Götze, D., Hämmerling, G. J., and Lemke, H.: Nomenclature for H-2 and la antigens defined by monoclonal antibodies. Immunogenetics 9: 503–507, 1979
Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497, 1975
Ledbetter, J. A., Goding, J. W., Tsu, T. T., and Herzenberg, L. A.: A new mouse lymphoid alloantigen (Lgp100) recognized by a monoclonal rat antibody. Immunogenetics 8: 347–360, 1979
Lemke, H., Hammerling, G. J., and Hammerling, U.: Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Qa/TL region in mice. Immunol. Rev. 47: 175–206, 1979
Nakayama, E., Dippold, W., Shiku, H., Oettgen, H. F., and Old, L. J.: Alloantigen-induced T-cell proliferation: Lyt phenotype of responding cells and blocking of proliferation by Lyt antisera. Proc. Natl. Acad. Sci. U.S.A. 77: 2890–2894, 1980
Old, L. J. and Stockert, E.: Immunogenetics of cell surface antigens of mouse leukemia. Annu. Rev. Genet. 11: 127–160, 1977
Ouchterlony O. and Nilsson, L.-A.: Immunodiffusion and electrophoresis. In (ed.): D. M. Weir, (ed.): Handbook of Experimental Immunology, 3rd edit., Chapter 19, Blackwell, Oxford, 1978
Paige, C. J., Kincade, P. W., and Ralph, P.: Marine B cell leukemia line with inducible surface immunoglobulin expression. J. Immunol. 121: 641–647, 1978
Sato, H. and Boyse, E. A.: A new alloantigen expressed selectively on B cells: the Lyb-2 system. Immunogenetics 3: 565–572, 1976
Shen, F.-W., Spanondis, M., and Boyse, E. A.: Multiple alleles of the Lyb-2 locus. Immunogenetics 5: 481–484, 1977
Snell, G. D., Cherry, M., McKenzie, I. F. C., and Bailey, D. W.: Ly-4, a new locus determining a lymphocyte cell-surface alloantigen in mice. Proc. Natl. Acad. Sci. U.S.A. 70: 1108–1111, 1973
Tada, N. and Hammerling, U.: Secretion of either of a pair of immunoglobulins, IgM or IgX, in somatic hybrid cells derived by fusion of a B-cell lymphoma cell line carrying both immunoglobulin isotypes. Immunogenetics 11: 7–19, 1980
Tada, N., Kimura, S., Hatzfeld, A., and Hammerling, U.: Ly-m11: The H-3 region of mouse chromosome 2 controls a new surface alloantigen. Immunogenetics 11: 441–449, 1980a
Tada, N., Kimura, S., Hoffmann, M., and Hämmerling, U.: A new surface antigen (PC.2) expressed exclusively on plasma cells. Immunogenetics 11: 351–362, 1980b
Taylor, B. A., Bailey, D. W., Cherry, M., Riblet, R., and Weigert, M.: Genes for immunoglobulin heavy chain and serum prealbumin protein are linked in mouse. Nature 256: 644–646, 1975
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The prefix m (monoclonal) is used following a suggestion by Klein and co-workers (1979).
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Tada, N., Kimura, S., Liu, Y. et al. Ly-m19: The Lyb-2 region of mouse chromosome 4 controls a new surface alloantigen. Immunogenetics 13, 539–546 (1981). https://doi.org/10.1007/BF00343721
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DOI: https://doi.org/10.1007/BF00343721