Abstract
Treatment of Saccharomyces cerevisiae cells with copper induces the activity of Cu/Zn superoxide dismutase (SOD) and catalase. To understand the level at which Cu regulates catalase, the expression of the S. cerevisiae CTA1 (encoding the peroxisomal catalase A) and CTT1 (encoding the cytosolic catalase T) genes was monitored as a function of Cu treatment. Copper was found to specifically induce transcription of CTT1, but not CTA1, mRNA. Moreover, genetic and biochemical studies demonstrate that this induction is independent of the ACE1 Cu trans-activator controlling the expression of yeast Cu/Zn SOD and metallothionein genes. Copper regulation of CTT1 thus appears to represent a novel metal regulatory pathway in S. cerevisiae cells.
Similar content being viewed by others
References
Aebi H (1984) Methods Enzymol 105:104–110
Belazzi T, Wagner A, Wieser R, Schanz M, Adam G, Hartig A, Ruis H (1991) EMBO J 10:585–592
Bostek CC (1989) J Am Ass Nurse Anes 57:213–237
Butt TR, Sternberg EJ, Gorman JA, Clark P, Hamer D, Rosenberg M, Crooke ST (1984) Proc Natl Acad Sci USA 81:3332–3336
Carri MT, Galiazzo F, Ciriolo MR, Rutilio G (1991) FEBS Lett 278:263–266
Cohen G, Fessl F, Traczyk A, Rytka J, Ruis H (1985) Mol Gen Genet 200:74–79
Culotta V, Hsu T, Hu S, Furst P, Hamer D (1989) Proc Natl Acad Sci USA 86:8377–8381
Davies K (1987) J Biol Chem 262:9895–9901
Fee JA (1982) Trends Biochem Sci 7:83–86
Feinberg AP, Vogelstein B (1983) Anal Biochem 132: 6–13
Fridovich I (1978) Science 201:875–880
Furst P, Hamer D (1989) Proc Natl Acad Sci USA 86:5267–5271
Furst P, Hu S, Hackett R, Hamer D (1988) Cell 55:705–717
Galiazzo F, Schiesser A, Rotilio G (1988) Biochim Biophys Acta 965:46–51
Galiazzo F, Ciriolo MR, Carri MT, Civitareale P, Marcocci L, Marmocchi F, Rotilio G (1991) Eur J Biochem 196:545–549
Gralla EB, Thiele DJ, Silar P, Valentine J (1991) Proc Natl Acad Sci USA 88:8558–8562
Greco M, Hrab D, Magner W, Kosman D (1990) J Bacteriol 172:317–325
Halliwell B (1982) Trends Biochem Sci 7:270–272
Hamer DH, Thiele D, Lemontt J (1985) Science 228:685–689
Hortner H, Ammerer G, Hartter E, Hamilton B, Rytka J, Bilinski T, Ruis H (1982) Eur J Biochem 128:179–184
Imlay J, Linn S (1988) Science 240:1302–1305
Kohrer K, Domdey H (1991) Methods Enzymol 194:398–415
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Michaelis S, Herskowitz I (1988) Mol Cell Biol 8:1309–1318
Ng A, Abelson J (1980) Proc Natl Acad Sci USA 77:3912–3916
Sambrook J, Fritsch E, Maniatis T (1989) In Molecular cloning. Laboratoy Cold Spring Harbor, New York
Simon M, Adam G, Rapatz W, Spevak W, Ruis H (1991) Mol Cell Biol 11:699–704
Skoneczny M, Chelstowska A, Rytka J (1988) Eur J Biochem 174:297–302
Spevak W, Fessl F, Rytka J, Traczyk A, Skoneczny M, Ruis H (1983) Mol Cell Biol 3:1545–1551
Thiele DJ (1988) Mol Cell Biol 8:2745–2752
Trush MA, Kensler T (1991) Free Rad Biol Med 10:201–209
Welch J, Fogel S, Buchman C, Karin M (1989) EMBO J 8:255–260
Wieser R, Adam G, Wagner A, Schuller C, Marchler G, Ruis H, Krawiec Z, Bilinski T (1991) J Biol Chem 266:12406–12411
Author information
Authors and Affiliations
Additional information
Communicated by R.J. Schweyen
Rights and permissions
About this article
Cite this article
Lapinskas, P., Ruis, H. & Culotta, V. Regulation of Saccharomyces cerevisiae catalase gene expression by copper. Curr Genet 24, 388–393 (1993). https://doi.org/10.1007/BF00351846
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00351846