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Multiple allelic states of the cyh2 gene cause low- and high-level cycloheximide resistance in Saccharomyces cerevisiae

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Summary

At least four different mutations at the cyh2 locus (rp1X; gene product: YL24) of Saccharomyces cerevisiae confer cycloheximide resistance. The mutant YL24 proteins are either more basic (high-level resistant phenotype), more acidic (low-level resistant phenotype), or unchanged in their electrophoretic mobility (both low-and high-level resistant phenotypes). None of the mutations at other loci seem to induce high-level resistance to cycloheximide.

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Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie der Universität, Maria-Ward-Str. 1a, 8000, München 19, Germany

    Walter Stöcklein & Wolfgang Piepersberg

  2. Instituto de Bioquimia de Macromoleculas, CSIC and UAM, Canto Blanco, Madrid-34, Spain

    Antonio Jiménez

  3. Department of Biochemistry, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Barbara Littlewood

  4. Biogen S.A., 3 Route de Troinex, 1227, Carouge, Switzerland

    Julian Davies

Authors
  1. Walter Stöcklein
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  2. Antonio Jiménez
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  3. Barbara Littlewood
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  4. Wolfgang Piepersberg
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  5. Julian Davies
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Stöcklein, W., Jiménez, A., Littlewood, B. et al. Multiple allelic states of the cyh2 gene cause low- and high-level cycloheximide resistance in Saccharomyces cerevisiae . Current Genetics 5, 157–160 (1982). https://doi.org/10.1007/BF00365708

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  • DOI: https://doi.org/10.1007/BF00365708

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