Abstract
Proteins were extracted from wheat meal or flour in 0.1 M acetic acid, 3 M urea and 0.01 M CTAB and fractionated in columns of cross-linked Sepharose in the same solvent. An heterogeneous fraction of high molecular weight eluted from the column which, when reduced and subjected to SDS-polyacrylamide-gel electrophoresis, separated into 12 components. Their molecular weights ranged from about 31,500 to 136,000. The unreduced protein was insoluble in salt solutions and aqueous ethanol but soluble in 0.1 M acetic acid and was therefore defined as glutenin. Glutenins of different molecular weight were made up from the same subunits but in different proportions. The ethanol-soluble proteins (gliadins) of the flour were fractionated in Sephadex G-100. The protein component that was excluded from the Sephadex gel, often described as high-molecular-weight gliadin, was shown to contain 8 distinguishable subunits and they had identical mobilities to 8 of the 12 subunits of glutenin.
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Abbreviations
- CTAB:
-
cetyltrimethyl ammonium bromide
- SDS:
-
sodium dodecyl sulphate
- AUC:
-
0.1 M acetic acid, 3 M urea and 0.01 M cetyltrimethyl ammonium bromide
References
Beckwith, A.C., Nielson, H.C., Wall, J.S., Huebner F.R.: Isolation and characterisation of a high-molecular-weight protein from wheat gliadin. Cereal Chem. 43, 14–28 (1966)
Bietz, J.A., Wall, J.S.: Wheat gluten subunits: molecular weights determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Cereal Chem. 49, 416–430 (1972)
Bietz, J.A., Wall, J.S.: The effect of various extractants on the subunit composition and associations of wheat glutenin. Cereal Chem. 52, 145–155 (1975)
Bramhall, S., Noack, N., Wu, M., Loewenberg, J.R.: A simple colorimetric method for determination of protein. Anal. Biochem. 31, 146–148 (1969)
Crow, M.J.A., Rothfus, J.A.: Chromatography of proteins from wheat gluten in polyacrylamide gel. Cereal Chem. 45, 413–420 (1968)
Danno, G., Kanazawa, K., Natake, M.: Extraction of wheat flour proteins with sodium dodecyl sulphate and their molecular weight distribution. Agr. Biol. Chem. 38, 1947–1953 (1974)
Hamauzu, Z., Arakawa, T., Yonezawa, D.: Molecular weights of glutenin and gliadin polypeptides estimated by SDS-polyacrylamide gel electrophoresis. Agr. Biol. Chem. 36, 1829–1830 (1972)
Huebner, F.R.: Comparative studies on glutenins from different classes of wheat. Agric. Food Chem. 18, 256–259 (1970)
Huebner, F.R., Wall, J.S.: Fractionation and quantitative differences of glutenin from wheat varieties varying in baking quality. Cereal Chem. 53, 258–269 (1976)
Jones, R.W., Babcock, G.E., Taylor, H.W., Senti, F.R.: Molecular weights of wheat gluten fractions. Arch. Biochem. Biophys. 94, 483–488 (1961)
Kasarda, D.D., Bernardin, J.E. and Nimmo, C.C.: Wheat proteins. In: Advances in Cereal Science and Technology, vol. I, pp. 158–236, Pomeranz, Y., ed. St. Paul, Minn.: Am. Assoc. Cereal Chem. 1976
Kanazawa, H., Yonezawa, D.: Studies on polypeptide composition of low molecular weight glutenin. Nippon Nogeikagau Kaishi 47, 17–22 (1973)
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970)
Meredith, O.B., Wren, J.J.: Determination of molecular-weight distribution in wheat-flour proteins by extraction and gel filtration in a dissociating medium. Cereal Chem. 43, 169–186 (1966)
Nielson, H.C., Beckwith, A.C., Wall, J.S.: Effect of disulphide bond cleavage on wheat gliadin fractions obtained by gel filtration. Cereal Chem. 45, 37–47 (1968)
Orth, R.A., Bushuk, W.: Studies of glutenin. 1. Comparison of preparative methods. Cereal Chem. 50, 106–114 (1973)
Osborne, T.B.: The proteins of the wheat kernel, Washington, D.C.: Carnegie Inst. 1907
Patey, A.L., Evans, D.J.: Large-scale preparation, of gliadin proteins. J. Sci. Fd. Agric. 24, 1229–1233 (1973)
Shearer, G., Patey, A.L., McWeeny, D.J.: Wheat flour proteins: the selectivity of solvents and the stability of gliadin and glutenin fractions of stored flours. J. Sci. Fd. Agric. 26, 337–344 (1975)
Wasik, R.J., Bushuk, W.: Studies of glutenin V. Note on additional preparative methods. Cereal Chem. 51, 112–118 (1974)
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Payne, P.I., Corfield, K.G. Subunit composition of wheat glutenin proteins, isolated by gel filtration in a dissociating medium. Planta 145, 83–88 (1979). https://doi.org/10.1007/BF00379931
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DOI: https://doi.org/10.1007/BF00379931