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Enzymes and coenzymes of the carbon monoxide dehydrogenase pathway for autotrophic CO2 fixation in Archaeoglobus lithotrophicus and the lack of carbon monoxide dehydrogenase in the heterotrophic A. profundus

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Abstract

Archaeoglobus lithotrophicus is a hyperthermophilic Archaeon that grows on H2 and sulfate as energy sources and CO2 as sole carbon source. The autotrophic sulfate reducer was shown to contain all the enzyme activities and coenzymes of the reductive carbon monoxide dehydrogenase pathway for autotrophic CO2 fixation as operative in methanogenic Archaea. With the exception of carbon monoxide dehydrogenase these enzymes and coenzymes were also found in A. profundus. This organism grows lithotrophically on H2 and sulfate, but differs from A. lithotrophicus in that it cannot grow autotrophically: A. profundus requires acetate and CO2 for biosynthesis. The absence of carbon monoxide dehydrogenase in A. profundus is substantiated by the observation that this organism, in contrast to A. lithotrophicus, is not mini-methanogenic and contains only relatively low concentrations of corrinoids.

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Abbreviations

F 420 :

coenzyme F420

MFR :

methanofuran

CHO-MFR :

formylmethanofuran

H 4MPT:

5,6,7,8-tetrahydromethanopterin

CHO−H 4MPT N5 :

formyl-H4MPT

CH≡H4MPT+N5 :

methenyl-H4MPT

CH 2=H4MPT N5, N10 :

methylene-H4MPT

CH 3−H4MPT N5 :

methyl-H4MPT

H 4F:

tetrahydrofolate

I U :

1 μmol/min

t d :

doubling time

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Authors and Affiliations

  1. Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität und Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35043, Marburg, Germany

    Julia Vorholt, Jasper Kunow & Rudolf K. Thauer

  2. Lehrstuhl für Mikrobiologie, Universität Regensburg, Universitätsstrasse 33, D-93053, Regensburg, Germany

    Karl O. Stetter

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  1. Julia Vorholt
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  2. Jasper Kunow
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  3. Karl O. Stetter
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  4. Rudolf K. Thauer
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Vorholt, J., Kunow, J., Stetter, K.O. et al. Enzymes and coenzymes of the carbon monoxide dehydrogenase pathway for autotrophic CO2 fixation in Archaeoglobus lithotrophicus and the lack of carbon monoxide dehydrogenase in the heterotrophic A. profundus. Arch. Microbiol. 163, 112–118 (1995). https://doi.org/10.1007/BF00381784

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