Summary
Seventy-six male volunteers, who were not occupationally exposed to polycyclic aromatic hydrocarbons (PAHs), participated in a study on the effect of tobacco smoking, alcohol consumption, dietary PAH intake, age, and body fat content on the baseline excretion of 1-hydroxypyrene in urine. Major determinants of urinary 1-hydroxypyrene excretion were smoking, dietary PAH intake, and age. The mean 1-hydroxypyrene concentrations in the urine of the volunteers in this study ranged between 0.05 and 0.79 μmol/mol creatinine. Smokers excreted on average 0.25 μmol/mol creatinine (range: 0.10–0.79 μmol/mol creatinine), and nonsmokers on average 0.12 μmol/mol creatinine (range: 0.04–0.29 μmol/mol creatinine). The average number of cigarettes smoked per day correlated well with urinary 1-hydroxypyrene concentrations (r s = 0.67, P < 0.001). The consumption of PAH-containing food products and active smoking account for 99% of total pyrene intake. The effect of age on 1-hydroxypyrene excretion is probably caused by a lower creatinine excretion in the elderly. Passive smoking and fat content had a statistically significant, but negligible effect on urinary 1-hydroxypyrene excretion. Passive smoking and the inhalation of ambient air are relatively inimportant for total pyrene intake (both account for less than 1%). Neither the consumption of alcohol nor the inhalation of ambient air significantly affected urinary 1-hydroxypyrene excretion. It is concluded that when urinary 1-OH-pyrene excretion is used in the assessment of PAH exposure, one should particularly be aware of the interindividual variability of the baseline excretion of PAH metabolites due to tobacco smoking and dietary PAH intake.
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References
Alessio L, Berlin A, Dell'Orto A, Toffoletto F, Ghezzi I (1985) Reliability of urinary creatinine as a parameter used to adjust values of urinary biological indicators. Int Arch Occup Environ Health 55:99–106
Alvarez E (1990) Le 1-hydroxypyren tant que marqueur de exposition aux hydrocarbures aromatiques polycycliques, Etudes preliminaires. SMIB Bruxelles, Belgium
Berlin A, Alessio L, Sesana G, Del'Orto A, Ghezzi I (1985) Problems concerning the usefulness of adjustment of urinary cadmium for creatinine and specific gravity. Int Arch Occup Environ Health 55:107–111
Bjorseth A, Becher G (1986) PAH in work atmospheres: occurrence and determination. CRC Press, Boca Raton, Fl.
Buchet JP, Gennart JP, Mercado-Calderon F, Delavignette JP, Cupers L, Lauwerys R (1992) Evaluation of exposure to polycyclic aromatic hydrocarbons in a coke production and a graphite electrode manufacturing plant: assessment of urinary excretion of 1-hydroxypyrene as a biological indicator of exposure. Br J Ind Med 49:761–768
Buckley TJ, Lioy PJ (1992) An examination of the time course from human dietary exposure to polycyclic aromatic hydrocarbons to urinary elimination of 1-hydroxypyrene. Br J Ind Med 49:113–1240
Burgaz S, Borm PJA, Jongeneelen FJ (1992) Evaluation of urinary excretion of 1-hydroxypyrene and thioethers in workers exposed to bitumen fumes. Int Arch Occup Environ Health 63:397–401
Butler JD, Crossley P (1979) An appraisal of relative airborne suburban concentrations of polycyclic aromatic hydrocarbons monitored indoors and outdoors. Sci Total Environ 11:53–58
Celier C, Cresteil T (1989) Induction of drug-metabolizing enzymes in Gunn rat liver, effect of polycyclic aromatic hydrocarbons on cytochrome P-450 regulation. Biochem Pharmacol 38:2825–2832
Clonfero E, Zordan M, Vemer P, Paleologo M, Levis AG, Cottica D, Pozzoli L, Jongeneelen FJ, Bos RP, Anzion RBM (1989) Biological monitoring of human exposure to coal tar urinary excretion of total polycyclic aromatic hydrocarbons, 1-hydroxypyrene and mutagens in psoriatic patients. Int Arch Occup Environ Health 61:363–368
Dennis MJ, Massey RC, Cripps G, Venn I, Howarth N, Lee G (1991) Factors affecting the polycyclic aromatic hydrocarbon content of cereals, fats and other food products. Food Addit Contam 8:517–530
De Vos RH, Van Dokkum W, Schouten A, De Jong-Berkhout P (1990) Polycyclic aromatic hydrocarbons in Dutch total diet samples (1984–1986). Food Chem Toxicol 28:263–268
EPA (1989) Exposure factors handbook. EPA/600/8-89/043, Environmental Protection Agency, Office of Health and Environmental Assessment, Washington D.C.
Gezondheidsraad (Dutch Health Council) (1984) Advies inzake Polycyclische Aromatische Koolwaterstoffen (PAK) in de buitenlucht. no: 40, 's-Gravenhage, The Netherlands
Grimmer G, Naujack K, Dettbarn G (1987) Gaschromatographic determination of polycyclic aromatic hydrocarbons, aza-arenes, aromatic amines in the particle and vapor phase of mainstream and sidestream smoke of cigarettes. Toxicol Lett 35:117–124
Gurtoo HL, Bejba Parker N (1977) Sex-dependent regulation of benzo[α]pyrene and zoxazolamine metabolism in rat tissues. Drug Metab Dispos 8:474–481
Hemminki K (1990) Environmental carcinogens. In: Cooper CS, Grover PL (eds) Chemical carcinogenesis and mutagenesis, vol I. Raven Press, New York, pp 33–62
Holtzman JL, Gebhard RL, Eckfeldt JH, Mottonen LR, Finley DK, Eshelman FN (1985) The effects of several weeks of ethanol consumption on ethanol kinetics in normal men and women. Clin Pharmacol Ther 38:157–163
IARC (1983) IARC monographs on the evaluation of carcinogenic risks to humans. Polynuclear Aromatic compounds, part l, vol 32. Lyon, France
IARC (1986) IARC monographs on the evaluation of carcinogenic risks to humans Tobacco smoking, vol 38. Lyon, France
IARC (1988) IARC monographs on the evaluation of carcinogenic risks to humans. Alcohol drinking, vol 44. Lyon, France
IARC (1991) IARC monographs on the evaluation of carcinogenic risks to humans. Coffee, tea, mate, methylxanthines and methylglyoxal, vol 51. Lyon, France
Jongeneelen FJ, Anzion RBM, Henderson P (1987) Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine. J Chromatogr 413:227–232
Jongeneelen FJ, Anzion RBM, Scheepers PTJ, Bos RP, Henderson P, Nijenhuis EH, Veenstra SJ, Brouns RME, Winkes A (1988a) 1-Hydroxypyrene in urine as a biological indicator of exposure to polycyclic aromatic hydrocarbons in several work environments. Ann Occup Hyg 32:35–43
Jongeneelen FJ, Bos RP, Henderson PT (1988b) Metabolites of polycyclic aromatic hydrocarbons in urine of exposed workers. Toxicol Environ Chem 16:295–307
Jongeneelen FJ, Scheepers PTJ, Groenendijk A, van Aerts LAGJ, Anzion RBM, Bos RP, Veenstra SJ (1988c) Airborne concentrations, skin contamination and urinary metabolite excretion of polycyclic aromatic hydrocarbons among paving workers exposed to coal tar derived road tars. Am Ind Hyg Assoc J 49:600–607
Jongeneelen FJ, Van Leeuwen FE, Oosterink S, Anzion RBM, Van der Loop F, Bos RP, Van Veen HG (1990) Ambient and biological monitoring of cokeoven workers, determinants of the internal dose of polycyclic aromatic hydrocarbons. Br J Ind Med 47:454–461
Kato R (1987) Sex-related difference in enzyme induction. In: Sotaniemi EA, Pelkonen O (eds) Enzyme induction in man. Taylor and Francis, London pp 47–60
Keuringsdienst van Waren (1990) Teer en nicotinegehalte in de rook van (shag)sigaretten, Alkmaar, The Netherlands
LAC-Milieu kritische stoffen (Dutch Agricultural Advise Committee) (1986) Polycyclische Aromatische Koolwaterstoffen in de Nederlandse voeding. LACnr: 86.3, 's-Gravenhage, The Netherlands
Li T, Bosron WF (1987) Distribution and properties of human alcohol dehydrogenase isoenzymes. Ann NY Acad Sci 492:1–10
Lieber CS (1990a) Interaction of ethanol with drugs, hepatotoxic agents, carcinogens and vitamins. Alcohol 25:157–176
Lieber CS (1990b) Interaction of alcohol with other drugs and nutrients: implication for the therapy of alcoholic liver disease. Drugs 40 (Suppl 3):23–44
Lieber CS (1990c) Mechanism of ethanol induced hepatic injury. Pharmacol Ther 46:l-41
Lieber CS, Garro A, Leo MA, Mak KM, Warner T (1986) Alcohol and cancer. Hepatology 6:1005–1019
Lieber CS, Lasker JM, Alderman J, Leo MA (1987) The microsomal ethanol oxidizing system and its interaction with other drugs, carcinogens, and vitamens. Ann NY Acad Sci 492:11–23
Lijinsky W (1991) The formation and occurrence of polynuclear aromatic hydrocarbons associated with food. Mutat Res 259:251–261
Lo MT, Sandi E (1978) Polycyclic aromatic compounds in food. In: Gunther FA (ed) Residue reviews. Residues of pesticides and other contaminants in the total environment. Springer, New York, pp 35–87
Papke J (1990) Neuere Aspekte zur Pharmacokinetik des Ethanols. Z Gesamte Inn Med 45:616–620
Pelkonen O, Pasanen M, Kuha H, Gachalyi B, Kairaluoma M, Sotaniemi EA, Park SS, Friedman FK, Gelboin HV (1986) The effect of cigarette smoking on 7-ethoxyresorufin O-deethylase and other monooxygenase activities in human liver: analysis with monoclonal antibodies. Br J Clin Pharmacol 22:125–134
Petruzzelli S, Camus A-M, Carossi L, Ghelarducci L, Rindi M, Menconi G, Angeletti CA, Ahotupa M, Hietanen E, Aitio A, Saracci R, Bartsch H, Giuntini C (1988) Long-lasting effects of tobacco smoking on pulmonary drug-metabolizing enzymes: a case-control study on lung cancer patients. Cancer Res 48:4695–4700
RIVM (Dutch Institute of Public Health and Environmental Protection) (1989) Integrated criteria document: polynuclear aromatic hydrocarbons. Report no.: 758474007. Bilthoven, The Netherlands
Rubin E, Gang H, Misra PS, Lieber CS (1970) Inhibition of drug metabolism by acute ethanol intoxication; a hepatic microsomal mechanism. Am J Med 49:801–806
Scherer G, Conze C, Tricker AR, Adlkofer F (1992) Uptake of tobacco smoke constituents on exposure to environmental tobacco smoke (ETS). J Clin Invest 70:352–367
Schmucker DL, Woodhouse KW, Wang RK, Wynne H, James OF, McManus M, Kremers P (1990) Effects of age and gender on in vitro properties of human liver microsomal monooxygenases. Clin Pharmacol Ther 48:365–374
Sesardic D, Davies DS, Edwards R, Boobis AR (1987) Identification of the major isoenzyme of cytochrome P450 induced by cigarette smoking in man. In: Sotaniemi EA, Pelkonen O (eds) Enzyme induction in man. Taylor and Francis, London, pp 33–46
Sesardic D, Cole KJ, Edwards RJ, Davies DS, Thomas PE, Levin W, Boobis AR (1990a) The inducibility and catalytic activity of cytochromes P450c(P450IA1) and P450d(P450IA2) in rat tissues. Biochem Pharmacol 39:499–506
Sesardic D, Pasanen M, Pelkonen O, Boobis AR (1990b) Differential expression and regulation of members of the cytochrome P450IA gene subfamily in human tissues. Carcinogenesis 11:1183–1188
Sherson D, Sigsgaard T, Overgaard E, Loft S, Poulsen HE, Jongeneelen FJ (1992) Interaction of smoking, uptake of polycyclic aromatic hydrocarbons, and cytochrome P450IA2 activity among foundry workers. Br J Ind Med 49:197–202
Speer K, Horstman P, Steeg E, Kühn T, Montag A (1990) Zur Analytic von Polycyclen in Gemüseproben (analysis of polycyclic aromatic hydrocarbons in vegetable samples). Z Lebensm Unters Forsch 191:442–448
Tolos WP, Shaw PB, Lowry LK, MacKenzie BA, Deng JF, Markel HL (1990) 1-Pyrenol: a biomarker for occupational exposure to polycyclic aromatic hydrocarbons. Appl Occup Environ Hyg 5:303–309
Vaessen HAMG, Schuller PL, Jekel AA, Wilbers AAMM (1984) Polycyclic aromatic hydrocarbons in selected foods; analysis and occurrence. Toxicol Environ Chem 7:297–324
Vaessen HAMG, Jekel AG, Wilbers AAMM (1988) Dietary intake of polycyclic aromatic hydrocarbons. Toxicol Environ Chem 16:281–294
Vähäkangas K, Pasanen M, Sotaniemi EA, Pelkonen O (1987) Drug metabolism in man: life-style factors: smoking and alcohol. In: Sotaniemi EA, Pelkonen O (eds) Enzyme induction in man. Taylor and Francis, London, pp 189–198
Van de Weerdt DHJ, Jongeneelen FJ (1991) Opname von polycyclische aromatische koolwaterstoffen door windsurfers (in Dutch, abstract in English). T Soc Gezondheidsz 69:466–472
Van de Wiel JAG, Meuwissen M, Kooy H, Fijneman PHS, Noordhoek J, Bos RP (1992) Influence of long-term ethanol treatment on in vitro biotransformation of benzo[α]pyrene in microsomes of the liver, lung and small intestine from male and female rats. Biochem Pharmacol 44:1977–1984
VanRooij JGM, Bodelier-Bade MM, de Loof AJA, Dijkmans APG, Jongeneelen FJ (1992) Dermal exposure to polycyclic aromatic hydrocarbons among primary aluminium workers. Med Lav 83:519–529
VanRooij JGM, Bodelier-Bade MM, Jongeneelen FJ (1993a) Estimation of individual dermal and respiratory uptake of polycyclic aromatic hydrocarbons in 12 coke oven workers. Br J Ind Med 50:623–632
Van Rooij JGM, Van Lieshout EMA, Bodelier-Bade MM, Jongeneelen FJ (1993b) Effect of the reduction of skin contamination on the internal dose of creosote workers exposed to polycyclic aromatic hydrocarbons. Scand J Work Environ Health 19:200–207
Zhao ZH, Quan WY, Tian DH (1992) Experiments on the effects of several factors on the 1-hydroxypyrene level in human urine as an idicator of exposure to polycyclic aromatic hydrocarbons. Sci Total Environ 113:197–207
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Van Rooij, J.G.M., Veeger, M.M.S., Bodelier-Bade, M.M. et al. Smoking and dietary intake of polycyclic aromatic hydrocarbons as sources of interindividual variability in the baseline excretion of 1-hydroxypyrene in urine. Int. Arch Occup Environ Heath 66, 55–65 (1994). https://doi.org/10.1007/BF00386580
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DOI: https://doi.org/10.1007/BF00386580