Summary
Biotransformation of NO, nitrite and nitrate was investigated in rats and mice in a 15NO inhalation experiment and intraperitoneal injection experiments of 15N-nitrite and 15N-nitrate, and the following results were obtained:
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(1)
Rats were forced to inhale 15NO (145 ppm,123 minutes) or were given an intraperitoneal injection of 15N-nitrite (2 mg animal−1 as 15N) or 15N-nitrate (2mg animal−1 as 15N), and determination of 15N recovery in urine was made up to 48 h later. The results were 55, 53 and 78% of the inhaled or injected 15N, respectively.
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(2)
15N-nitrate in the urine was converted into a 6-nitro derivative of 3,4-xylenol and its identification and quantitative determination were made by the GGMS method. As to 15N-urea in the urine, identification and quantitative determination were made by the urease method. 15N was present in the urine of rats after 15NO inhalation in the form of N03 − and urea. 75 and 24% respectively. In the urine of rats injected with 15N-nitrite, about 20% of unidentified 15N-compounds not discovered in the inhalation experiment was found. The content of 15N-urea in the urine after injection with 15N-nitrate was lower than that after injection with 15N-nitrite.
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(3)
When 15N-nitrite (0.617 mg animal−1 as 15N) was injected intraperitoneally in mice, 60.7, 7.8 and 0.3% of the injected 15N were found in the urine, feces and exhaled gas (NO, N02 and NH3 in the gas were caught) up to 48 h after injection respectively, and 1.6% was found in the body 48 h after injection, but the remaining 30% of 15N could not be recovered.
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Yoshida, K., Kasama, K., Kitabatake, M. et al. Biotransfornation of nitric oxide, nitrite and nitrate. Int. Arch Occup Environ Heath 52, 103–115 (1983). https://doi.org/10.1007/BF00405415
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DOI: https://doi.org/10.1007/BF00405415