Summary
During formation of fungal mats of Cunninghamella sp., colchicine seemed to have no effect on the mycelial dry weight but increased the phosphorus and nitrate uptake only when present in 20 p.p.m. All treatments increased the organic phosphorus content and peptide fraction of the tissue medium systems. These phenomena were furthered by raising the concentration of the drug. Colchicine showed its inhibitory effects on protein synthesis only when administered in the high concentration. The mechanism of nitrate utilisation in presence of colchicine was also discussed.
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Barr, C. G., and E. H. Newcomen: Physiological aspects of tetraploid cabbage. J. Agr. Res. 67, 321 (1943).
Chen, S. L., and P. S. Tang: Colchicine induced tetraploid barley; 1. Physiological study. Amer. J. Bot. 32, 103 (1945).
——: Colchicine induced tetraploid barley; 2. Enzyme activities. Amer. J. Bot. 32, 180 (1945).
Chibnall, A. C.: Protein Metabolism in the Plant. Yale Univ. Press 1939.
Chopra, R. N., and K. S. Rai: Response of ovules of Argemone mexicana to colchicine treatment in vivo. Phytopathology (Delhi) 8, 107 (1958).
Delory, G. E.: Photoelectric Methods in Clinical Biochemistry. p. 76 (1949).
Eckerson, S. H.: Protein synthesis by plants; 1. Nitrate reduction. Bot. Gaz. 77, 377 (1924).
Ekdahl, I.: Ark. Bot. (Stockh.) 13A, 1 (1944). In: Naguib, M. I.: M. Sc. Thesis Cairo University 1949.
Evans, H. S., and A. Nason: Pyridine nucleotide nitrate reductase from extracts of higher plants. Plant Physiol. 28, 233 (1953).
Fiske, C. H., and Y. Subbarrow: The colorimetric determination of phosphorus. J. biol. Chem. 66, 375 (1925).
Friedman, N. B.: Effect of chemotherapy and irradiation therapy on the differentiation of experimental tumors. Cancer 11 (5), 1060 (1958).
Koga, M.: Starch synthesis in sweet potato in sterile culture. Physiol. and Ecol. (Jap.) 7 (2), 87 (1957).
Loo, T. L.: A comparison of the effects of plant growth of microelements, indoleacetic acid and colchicine. Ann. Bot. N. S. 5 (8), 357 (1944).
Naguib, K.: Growth and metabolism of Aspergillus nidulans Eidam in surface culture. Canad. J. Bot. 37, 353 (1959).
—: Growth and metabolism of Penicillium lilacinum Thom. with reference to the effects of riboflavin and nicotinic acid. Arch. Mikrobiol. 35, 296 (1960).
Naguib, M. I.: M. Sc. Thesis Cairo University. (1949).
Naguib, M. I.: A rapid colorimetric procedure for the estimation of free and conjugated sugars in plant extracts. Z. Zucker 15 (17), 351 (1962).
Nason, A., R. G. Abraham and B. C. Averback: The enzymatic reduction by reduced pyridine nucleotides. Biochim. biophys. Acta (Amst.) 15, 160 (1954).
—, and H. J. Evans: Triphosphopyridine nucleotide nitrate reductase in Neurospora. J. biol. Chem. 202, 655 (1954).
Nicholas, D. J. D., and A. Nason: Mechanism of action of nitrate reductase from Neurospora. J. biol. Chem. 221, 183 (1954).
Nickell, L. G.: Effect of certain plant hormones and colchicine on the growth and respiration of virus tumor tissues from Rumex acetosa. Amer. J. Bot. 37 (10), 829 (1950).
Noguti, Y., H. Oka and T. Otuka: Polyploidy in Nicotiana induced by treatment with colchicine. Growth rate and chemical analysis of diploid and its autotetraploid Nicotiana Rustica and Nicotiana tabacum. Japan. J. Bot. 10, 343 (1940).
Paech, K., and M. V. Tracey: Modern Methods of Plant Analysis, Vol. I, p. 481. Berlin, Göttingen, Heidelberg: Springer 1955.
Russel, J. A.: Colorimetric detection of amino nitrogen. J. biol. Chem. 15, 567 (1944).
Said, H., and K. Naguib: Effect of 2:4-Dichlorophenoxyacetic acid on absorption and assimilation of sucrose by Fusarium moniliforme Sheldon. Bull. Fac. Sci. Cairo Univ. 38, 49 (1962).
Salama, A. M., and M. I. Naguib: Effect of colchicine on the carbohydrate metabolism of mycelial felts of Cunninghamella sp. Arch. Mikrobiol. 46, 296 (1963).
Tamiya, H., Y. Morimura and M. Yokota: Effect of various antimetabolites upon the life cycle of Chlorella. Arch. Mikrobiol. 42, 4 (1962).
Zucker, M., and A. Nason: Enzymatic reduction of hydroxylamine to ammonia by reduced pyridine nucleotides. Fed. Proc. 13, 328 (1954).
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Naguib, M.I. Effect of colchicine on the nitrogen metabolism and fate of phosphates absorbed during the formation of fungal felts of cunninghamella sp.. Archiv. Mikrobiol. 47, 154–160 (1963). https://doi.org/10.1007/BF00422520
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DOI: https://doi.org/10.1007/BF00422520