Summary
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1.
The ultrastructural changes accompanying re-greening of “giant”, bleached cells of the Emerson strain of Chlorella have been studied.
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2.
Re-greening is induced either by transferring the cells from heterotrophic to autotrophic conditions (light recovery) or by allowing the cells to remain under heterotrophic conditions for a prolonged period (dark recovery).
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3.
During light recovery new thylakoids arise in such a way as to suggest their origin from degraded remnants of the original parental thylakoids.
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4.
During dark recovery, some of the thylakoids appear to arise from invaginations of the inner membrane of the chloroplast envelope.
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5.
In both light and dark recovery, chloroplast development is accompanied by extensive mobilization of the reserve starch grains and the de novo synthesis of pyrenoids in the chloroplasts of the autospores produced.
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6.
Chloramphenicol, which inhibits re-greening, does not suppress the formation of thylakoids during light recovery. In the presence of this antibiotic, however, there is no synthesis of chloroplast ribosomes and the thylakoids lack some component essential for their structural integrity and for their further association into lamellae.
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7.
Since both light and dark recovery of chloroplast development is acompanied by a recovery of cell division, previously held in check, the system is eminently suitable for studying the interrelationships between the chloroplast and the rest of the cell.
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Van Thinh, L., Griffiths, D.J. Ultrastructural changes accompanying the recovery of “giant” cells of the Emerson strain of Chlorella . Archiv. Mikrobiol. 87, 61–75 (1972). https://doi.org/10.1007/BF00424779
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DOI: https://doi.org/10.1007/BF00424779