Abstract
Methanobacterium thermoautotrophicum was grown in continuous culture in a fermenter gassed with H2 and CO2 as sole carbon and energy sources, and in a medium which contained either NH4Cl or gaseous N2 as nitrogen source. Growth was possible with N2. Steady states were obtained at various gas flow rates with NH4Cl and with \(N2{\text{ }} \cdot {\text{ Y}}_{{\text{CH}}{}_{\text{4}}} \) and the maintenance coefficient varied with the gas input and with the nitrogen source. Growth of Methanococcus thermolithotrophicus in continuous culture in a fermenter gassed with H2, CO2 as nitrogen, carbon and energy sources was also examined.
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Abbreviations
- \(Y_{CH_4 } \) :
-
molecular growth yield (g dry weight of cells per mol of CH4 evolved)
- μ:
-
growth rate (h-1)
- D :
-
dilution rate (h-1)
- rate (h-1); \(\frac{1}{{Y_{CH_4 } }} = \frac{1}{{Y_{\max {\text{ theor}}} }} + mg + \frac{{ms}}{\mu }\) :
-
relation of Neijssel and Tempest and of Stouthamer and Bettenhaussen
- \(E_1 = \left( {1 - \frac{{Y_{CH_4 }^{\max {\text{ exp}}} }}{{Y_{CH_4 }^{\max } }}} \right){\text{ x 100}}\) :
-
energy
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Fardeau, M.L., Peillex, J.P. & Belaïch, J.P. Energetics of the growth of Methanobacterium thermoautotrophicum and Methanococcus thermolithotrophicus on ammonium chloride and dinitrogen. Arch. Microbiol. 148, 128–131 (1987). https://doi.org/10.1007/BF00425360
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DOI: https://doi.org/10.1007/BF00425360