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Occurrence of histone-related oxalate binding in rat liver nucleus

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Abstract

The rat liver nuclear oxalate binding protein was isolated, purified by anion and cation exchange column chromatography using Diethyl Amino Ethyl Sephadex, Carboxy Methyl Cellulose and Carboxy Methyl Sephadex C-50 ion exchangers. The purified oxalate binding protein was found to be H1B of H1 fraction of histories. Kinetic analysis of oxalate binding showed the presence of two affinity sites, one with Kd of 133.5 nM and Bmax of 40 pmoles and another with Kd of 262.5 nM and Bmax of 210 pmoles. The optimal oxalate binding was at pH 4.2 and at 28°C. The oxalate binding was specific and reversible and not due to ionic charge interaction. The IC50 of other dicarboxylates was higher than that of oxalate. EGTA had no effect on oxalate binding but di- and tri-carboxylate carrier inhibitors and thiol modifying agents significantly lowered the binding activity. Oxalate binding to histones was significantly reduced in the presence of DNA or nucleotides, but RNA had no effect. ATP completely inhibited the oxalate binding activity at 1 mM concentration. Different tissues exhibited oxalate binding showing ubiquitous nature. Calf thymus H1 showed maximal binding similar to liver histones.

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Abbreviations

ADP:

Adenosine diphosphate

ATP:

Adenosine triphosphate

DNA:

Deoxyribonucleic acid

RNA:

Ribonucleic acid

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  1. Department of Medical Biochemistry, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, 600 113, Taramani, Madras, India

    Ramasamy Selvam & Vadlamudi Prasanna Lakshmi

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  1. Ramasamy Selvam
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  2. Vadlamudi Prasanna Lakshmi
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Selvam, R., Prasanna Lakshmi, V. Occurrence of histone-related oxalate binding in rat liver nucleus. Mol Cell Biochem 156, 93–100 (1996). https://doi.org/10.1007/BF00426330

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  • DOI: https://doi.org/10.1007/BF00426330

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