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An ultrastructural study of the invasion of Culex quinquefasciatus larvae by Leptolegnia chapmanii (Oomycetes: Saprolegniales)

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Abstract

Transmission electron microscopy of the invasion of Culex quinquefasciatus by Leptolegnia chapmanii confirmed that it is a primary pathogen and revealed several differences between penetration via the gut and penetration via the integument. The latter often involved aggregations of zoospores, appressoria-like swellings of the invasive hyphae, and lateral growth of hyphae between the epicutical and endocuticle. These features were not detected in the case of gut invasion, but hyphal septa at the point of entry were apparently peculiar to this route. There was no evident tissue specificity, and death presumably resulted from generalized destruction of tissues.

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References

  1. Aist JR. Cytology of penetration and infection — fungi. In: Heitenfuss R, Williams PH, eds. Encyclopedia of plant physiology, New Ser., Vol 4. Berlin: Springer-Verlag, 1976: 197–221.

    Google Scholar 

  2. Becnel JJ, Hazard EI, Fukuda T. Fine structure and development of Pirosporella chapmanii (Microspora: Thelohaniidae) in the mosquito Aedes triseriatus (Say). J Protozool 1986; 33:60–66.

    Google Scholar 

  3. Clark G. Staining procedures, 4th ed. Baltimore: Williams and Wilkins Co., 1981.

    Google Scholar 

  4. Clark TB, Kellen WR, Lindegren JE, Sanders RD. Pythium sp. (Phycomycetes: Pythiales) pathogenic to mosquito larvae. J Invertebr Pathol 1966; 8:351–54.

    Google Scholar 

  5. Clements AN. The physiology of mosquitoes. Oxford: Pergamon Press, 1963: 366.

    Google Scholar 

  6. Gill GW, Frost JK, Miller KA. A new formula for a halfoxidized hematoxylin solution that neither overstains nor requires differentiation. Acta Cytol 1974; 18:300–311.

    Google Scholar 

  7. Gotz P, Vey A. Humoral encapsulation in Diptera (Insecta): defence reactions of Chironomus larvae against fungi. Parasitology 1974; 68:193–205.

    Google Scholar 

  8. Kraft JM, Endo RM, Erwin DC. Infection of primary roots of bentgrass by zoospores of Pythium aphanidermatum. Phytopathology 1967; 57:86–90.

    Google Scholar 

  9. Lai-Foof J. The repair of wounds in the integument of insects. J Insect Physiol 1966; 12:195–226.

    Google Scholar 

  10. McInnis T Jr, Zattau WC. Experimental infection of mosquito larvae by a species of the aquatic fungus Leptolegnia. J Invertebr Pathol 1982; 39:98–104.

    Google Scholar 

  11. McInnis T Jr, Schimmel L, Noblet R. Host range studies with the fungus Leptolegnia, a parasite of mosquito larvae (Diptera: Culicidae). J Med Entomol 1985; 22:226–27.

    Google Scholar 

  12. Nnakumusana ES. Susceptibility of mosquito larvae to Leptolegnia sp. Indian J Med Res 1987; 84:586–93.

    Google Scholar 

  13. Pearse AGE. Histochemistry: Theoretical and applied, Vol. 2. Baltimore: Williams and Wilkins Co., 1972.

    Google Scholar 

  14. Poinar GO, Leutenegger R. Ultrastructural investigation of the melanization process in Culex pipiens (Culicidae) in response to a nematode. J Ultrastruct Res 1971; 36:149–58.

    Google Scholar 

  15. Richards AG, Richards PA. The peritrophic membrane of insects. Ann Rev Entomol 1977; 22:219–40.

    Google Scholar 

  16. Seymour RL. Leptolegnia chapmanii, an oomycete pathogen of mosquito larvae. Mycologia 1984; 76:670–74.

    Google Scholar 

  17. Shimony C, Friend J. The ultrastructure of the interaction between Phytophthora infestans (Mont.) deBary and tuber discs of potato (Solanum tuberosum L.) cv. King Edward. Physiol Plant Pathol 1977; 11:243–49.

    Google Scholar 

  18. Stiles KA. Normal butyl alcohol technic for animal tissues with special reference to insects. Stain Technol 1934; 9:97–100.

    Google Scholar 

  19. Zattau WC, McInnis T Jr. Life cycle and mode of infection of Leptolegnia chapmanii (Oomycetes) parasitizing Aedes aegypti. J Invertebr Pathol 1987; 50:134–45.

    Google Scholar 

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Lord, J.C., Fukuda, T. An ultrastructural study of the invasion of Culex quinquefasciatus larvae by Leptolegnia chapmanii (Oomycetes: Saprolegniales). Mycopathologia 104, 67–73 (1988). https://doi.org/10.1007/BF00436929

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