Abstract
Rhodopseudomonas capsulata was grown either phototropically in the light or chemotrophically in the dark at oxygen tensions of 5 mm and 3 mm Hg in ammonium-limited continuous culture. During growth limitation bacteriochlorophyll content of cells and membranes varied dependent on growth rate drastically in chemotrophic cultures. Concomittantly, the ratio of membrane protein to total protein varied in the range of 30–41%. This dependence of membrane differentiation on growth rate was less evident in phototrophically grown cells.
The incorporation of the bulk of bacteriochlorophyll was shown to be quantitatively correlated to the incorporation of 1–3 low molecular weight proteins with molecular weights in the range of 14 to less than 10 k daltons. Supported by similar findings of other authors it is proposed, that these proteins are to be attributed to the species of antenna bacteriochlorophyll and represent components of the photosynthetic apparatus. With decreasing growth rates the size of the photosynthetic unit with respect to the population of bacteriochlorophyll-and protein molecules was reduced subsequent to a reduction in the rate of incorporation of antenna-bacteriochlorophyll and the low molecular weight proteins, the reaction-center bacteriochlorophyll content of the membranes remaining constant. A parallel decrease in potential phosphorylating capacity was observed.
It is concluded, that under these conditions, primary photochemical reactions in the reaction center were not the rate-limiting step in photophosphorylation. The interaction of growth limitation by an anabolic precursor (NH +4 ) and control of membrane differentiation by light intensity or oxygen tension is discussed.
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References
Aagaard, J., Sistrom, W. R.: Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochem. Photobiol. 55, 209–225 (1972)
Baccarini-Melandri, A., Melandri, B. A.: Energy transduction in photosynthetic bacteria, III. coincidence of coupling factor of photosynthesis and respiration in Rhodopseudomonas capsulata. FEBS Letters 21, 131–134 (1972)
Biedermann, M., Drews, G., Marx, R., Schröder, J.: Der Einfluß des Sauerstoffpartialdrucks und der Antibiotica Actinomycin und Puromycin auf das Wachstum, die Synthese von Bakteriochlorophyll und die Thylakoidmorphogenese in Dunkelkulturen von Rhodospirillum rubrum. Arch. Mikrobiol. 56, 133–147 (1967)
Cavari, B., Kalra, V. K., Brodie, A. F.: Oxidative phosphorylation in fractionated bacterial systems: effect of chloramphenicol. J. Bact. 108, 1017–1025 (1971)
Clayton, R. K.: Photochemical reaction centers in photosynthetic tissues. In: Bacterial photosynthesis, H. Gest, A. San Pietro, L. P. Vernon, eds., p. 377. Yellow Springs: Antioch Press 1963
Clayton, R. K.: Molecular physics in photosynthesis, pp. 1–37. New York: Blaisdell 1965
Clayton, R. K., Clayton, B. J.: Relations between pigments and proteins in the photosynthetic membranes of Rhodopseudomonas spheroides. Biochim. biophys. Acta (Amst.) 283, 492–504 (1972)
Clayton, R. K., Haselkorn, R.: Protein components of bacterial photosynthetic membranes. J. molec. Biol. 68, 97–105 (1972)
Clayton, R. K., Sistrom, W. R.: An absorption band near 800 nm associated with P 870 in photosynthetic bacteria. Photochem. Photobiol. 5, 661–668 (1966)
Clayton, R. K., Wang, R. T.: Photochemical reaction center from Rhodopseudomonas spheroides. In: Methods in enzymology, Vol. 23, A. San Pietro, ed., pp. 696–704. New York-London: Academic Press 1971
Cohen-Bazire, G., Kunisawa, R.: Some observations on the synthesis and function of the photosynthetic apparatus in Rhodospirillum rubrum. Proc. nat. Acad. Sci. (Wash.) 46, 1543–1555 (1966)
Cusanovich, M. A., Kamen, M. D.: Light-induced electron transport in Chromatium strain D. III. Photophosphorylation by Chromatium chromatophores. Biochim. biophys. Acta (Amst.) 153, 418–426 (1968)
Dierstein, R., Drews, G.: Nitrogen-limited continuous culture of Rhodopseudomonas capsulata growing photosynthetically or heterotrophically under low oxygen tensions. Arch. Microbiol. 99, 117–128 (1974)
Drews, G.: Untersuchungen zur Regulation der Bacteriochlorophyllsynthese bei Rhodospirillum rubrum. Arch. Mikrobiol. 51, 186–198 (1965)
Drews, G., Dierstein, R., Nieth, K. F.: The formation of the photosynthetic apparatus in cells of Rhodopseudomonas capsulata. In: Proc. 3rd Int. Congr. Photosynthesis, Vol. 3, M. Avron, ed., pp. 2139–2146. Amsterdam: Elsevier (1974)
Feher, G., Okamura, M. Y., McElroy, J. D.: Identification of an electron acceptor in reaction centers of Rhodopseudomonas spheroides by EPR spectroscopy. Biochim. biophys. Acta (Amst.) 267, 222–226 (1972)
Fraker, P. J., Kaplan, S.: Isolation and characterization of a bacteriochlorophyll containing protein from Rhodopseudomonas spheroides). J. biol. Chem. 247, 2732–2737 (1972)
Goedheer, J. C.: Fluorescence polarization and pigment orientation in photosynthetic bacteria. Biochim. biophys. Acta (Amst.) 292, 665–676 (1973)
Huang, J. W., Kaplan, S.: Membrane proteins of Rhodopseudomonas spheroides, V. Additional chemical characterization of a pigment lipid associated protein isolated from chromatophores. Biochim. biophys. Acta (Amst.) 307, 332–342 (1973)
Irschik, H., Oelze, J.: Membrane differentiation in phototrophically grown Rhodospirillum rubrum during transition from low to high light intensity. Biochim. biophys. Acta (Amst.) 330, 80–89 (1973)
Ke, B., Chaney, T. H.: Spectral and photochemical properties of subchromatophore fraction derived from carotenoid deficient Chromatium by Triton-treatment. Biochim. biophys. Acta (Amst.) 226, 341–353 (1971)
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature (Lond.) 227, 680–685 (1970)
Lampe, H. H., Drews, G.: Die Differenzierung des Membransystems von Rhodopseudomonas capsulata hinsichtlich seiner photosynthetischen und respiratorischen Funktion. Arch. Mikrobiol. 84, 1–19 (1972)
Lampe, H. H., Oelze, J., Drews, G.: Die Fraktionierung des Membransystems von Rhodopseudomonas capsulata und seine Morphogenese. Arch. Mikrobiol. 83, 78–94 (1972)
Lien, S., Gest, H.: On the identity of photo- and oxidative phosphorylation coupling factors in Rhodopseudomonas capsulata. Arch. Biochem. Biophys. 159, 730–737 (1973)
Lien, S., Gest, H., San Pietro, A.: Regulation of chlorophyll synthesis in photosynthetic bacteria. Bioenergetics 4, 423–434 (1973)
Lien, S., San Pietro, A., Gest, H.: Mutational and physiological enhancement of photosynthetic energy conservation in Rhodopseudomonas capsulata. Proc. nat. Acad. Sci. (Wash.) 68, 1912–1915 (1971)
Lopez, R. M. Y., Siekevitz, P.: SDS polyacrylamide gel electrophoresis of membrane proteins: effect of phospholipids. Analyt. Biochem. 53, 594–602 (1973)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Nieth, K. F., Drews, G.: The protein patterns of intracytoplasmatic membranes and reaction center particles isolated from Rhodopseudomonas capsulata. Arch. Microbiol. 96, 161–174 (1974)
Nieth, K. F., Drews, G.: Formation of reaction center and light harvesting bacteriochlorophyll-protein complexes in Rhodopseudomonas capsulata. Arch. Microbiol. 104, 77–82 (1975)
Nieth, K. F., Drews, G., Feick, R.: Photochemical reaction centers from Rhodopseudomonas capsulata. Arch. Microbiol. 105, 43–45 (1975)
Oelze, J., Drews, G.: Membranes of photosynthetic bacteria. Biochim. biophys. Acta (Amst.) 265, 209–239 (1972)
Okamura, M. Y., Steiner, A., Feher, G.: Characterization of reaction centers from photosynthetic bacteria, I. Subunit structure of the protein mediating the primary photochemistry in Rhodopseudomonas spheroides. Biochemistry 13, 1394–1402 (1974)
Reed, D. W.: Isolation and composition of a photosynthetic reaction center complex from Rhodopseudomonas spheroides. J. biol. Chem. 244, 4936–4941 (1969)
Reed, D. W., Peters, G. A.: Characterization of the pigments in reaction center preparations from Rhodopseudomonas spheroides. J. biol. Chem. 247, 7148–7152 (1972)
Reed, D. W., Raveed, D., Israel, H. W.: Functional bacteriochlorophyll-protein complexes from chromatophores of Rhodopseudomonas spheroides strain R-20. Biochim. biophys. Acta (Amst.) 223, 281–291 (1970)
Schlecht, S., Ring, K., Kutscher, J., Eschweiler, W.: Ein neuer Laboratoriumsfermenter zur Züchtung von Mikroorganismen im turbidostatischen, chemostatischen und “batch”-Verfahren. Zbl. Bakt., I. Abt. Orig. 206, 246–258 (1968)
Schwenker, U., Gingras, G.: A carotenoprotein from chromatophores of Rhodospirillum rubrum. Biochem. biophys. Res. Commun. 51, 94–99 (1973)
Schwenker, U., St.-Onge, M., Gingras, G.: Chemical and physical properties of a carotenoprotein from Rhodospirillum rubrum. Biochim. biophys. Acta (Amst.) 351, 246–260 (1974)
Sistrom, W. R., Clayton, R. K.: Studies on a mutant of Rhodopseudomonas spheroides unable to grow photosynthetically. Biochim. biophys. Acta (Amst.) 88, 61–73 (1964)
Slooten, L.: Reaction center preparation of Rhodopseudomonas spheroides. Biochim. biophys. Acta (Amst.) 256, 452–466 (1972)
Smith, J. H., Benitez, A.: Chlorophylls. In: Moderne Methoden der Pflanzenanalyse, Vol. 4, K. Paech, M. V. Tracey, eds., p. 179. Berlin-Göttingen-Heidelberg: Springer 1955
Stebbing, N.: Feed-back control mechanism in microorganisms and efficiency of growth. Sub-cell. Biochem. 2, 169–182 (1973)
Takemoto, J.: Kinetics of photosynthetic membrane protein assembly in Rhodopseudomonas spheroides. Arch. Biochem. Biophys. 163, 515–520 (1974)
Takemoto, J., Lascelles, J.: Coupling between bacteriochlorophyll and membrane protein synthesis in Rhodopseudomonas spheroides. Proc. nat. Acad. Sci. (Wash.) 70, 799–803 (1973)
Tempest, D. W., Herbert, D.: Effect of dilution rate and growth limiting substrate on the metabolic activities of Torula utilis cultures. J. gen. Microbiol. 41, 143–150 (1965)
Vredenberg, W. J., Amesz, J.: Absorption bands of bacteriochlorophyll types in purple bacteria and the response to illumination. Biochim. biophys. Acta (Amst.) 126, 244–253 (1966)
Weber, K., Osborn, M.: The reliability of molecular weight determination by dodecylsulfate-polyacrylamide gel electrophoresis. J. biol. Chem. 244, 4406–4412 (1969)
Worden, P. B., Sistrom, W. R.: The preparation and properties of bacterial chromatophore fractions. J. Cell Biol. 23, 135–150 (1964)
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Dierstein, R., Drews, G. Control of composition and activity of the photosynthetic apparatus of Rhodopseudomonas capsulata grown in ammonium-limited continuous culture. Arch. Microbiol. 106, 227–235 (1975). https://doi.org/10.1007/BF00446528
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DOI: https://doi.org/10.1007/BF00446528