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Selection of gamma-ray mutants from a strain of Humicola lutea 72, producing acid proteases

  • Applied Microbiology
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

Gamma-ray exposure was used as a mutagenic agent for mutant induction from a strain of Humicola lutea 72 producing acid proteases. The second stage of irradiation resulted in a double active mutant (H. lutea 120–8). Further treatment gave rise to a triple active mutant (H. lutea 120–5) with a maximal proteolytic activity of 1,800 μg tyrosine liberated from 2% casein/ml−1 culture filtrate/min−1 at pH 3,0 and 40°C. A change in the composition of proteolytic enzymes caused by gamma-ray exposure was established, using chromatography on DEAE-cellulose.

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Authors and Affiliations

  1. Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    I. Grigorov, P. Aleksieva, A. Djerova & P. Sheremetska

  2. Institute of Organic Chemistry and Center of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    B. Tchorbanov

Authors
  1. I. Grigorov
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  2. P. Aleksieva
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  3. A. Djerova
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  4. P. Sheremetska
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  5. B. Tchorbanov
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Grigorov, I., Aleksieva, P., Djerova, A. et al. Selection of gamma-ray mutants from a strain of Humicola lutea 72, producing acid proteases. European J. Appl. Microbiol. Biotechnol. 17, 355–357 (1983). https://doi.org/10.1007/BF00499503

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  • DOI: https://doi.org/10.1007/BF00499503

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