Summary
Human fetal tissue fixed with Bouin and kept for 30 years in ethanol is still useful in immunohistochemistry. The localization of β-endorphin immunoreactive cells in the pituitary is given as example.
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References
Bégeot, M., Dubois, M.P., Dubois, P.M.: Immunologic localization of α- and β-endorphins and β-lipotropin in corticotropic cells of the normal and anencephalic fetal pituitaries. Cell Tiss. Res. 193, 413–422 (1978)
Celio, M.R. et al.: Manuscript in preparation
Halmi, N.S.: Immunostaining of growth hormone and prolactin in paraffin embedded and stored or previously stained material. J. Histochem. Cytochem. 26, 486–495 (1978)
Höllt, V., Przewlocki, R., Herz, A.: Radioimmunoassay of β-endorphin. Basal and stimulated levels in extracted rat plasma. Naunyn-Schmiedberger's Arch. Pharmacol. 303, 171–174 (1978)
Sternberger, L.A.: Immunocytochemistry. Englewood Cliffs, NJ: Prentice Hall 1974
Taylor, C.E., Burns, J.: The demonstration of plasma cells and other immunoglobulin containing cells in formalin fixed, paraffin embedded tissue using peroxydase labeled antibodies. J. Clin. Pathol. 27, 14–21 (1974)
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Celio, M.R. Immunohistochemistry on Bouin-fixed fetal tissue, stored for thirty years in ethanol. Histochemistry 61, 347–350 (1979). https://doi.org/10.1007/BF00508457
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DOI: https://doi.org/10.1007/BF00508457