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Evidence for presence of kynurenine in lung and brain of neonate hamsters

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Summary

Using immunocytochemical techniques, we report here direct evidence of kynurenine (Kyn) presence and localization in the lung and brain. Kyn is a metabolite of tryptophan and 5-hydroxytryptophan, produced by indoleamine 2, 3-dioxygenase (IDO). Whereas IDO has been quantitated in tissues from lung, brain, and other organs, Kyn has only been identified in brain (by HPLC), and its specific localization has not been determined.

We reacted alternate serial paraffin sections with anti-sera raised in rabbits against a l-Kyn-albumin conjugate, and with anti-5HT (serotonin, 5-hydroxytryptamine), using the PAP method. Kyn-like immunoreactivity in the lung was specifically localized to cells of the bronchiolar epithelium resembling basal cells. Taller epithelial cells in the bronchi and dorsal trachea were likewise positive whereas neuroepithelial bodies were negative. Immunoreactivity in the brain was typically localized to cells localized in the ependyma of the walls of all ventricles, and to nerve fibers. The cellular Kyn-like reactivity was totally separate from that of anti-5HT, the latter uniquely staining argyrophil lung neuroendocrine cells and raphae neurons of the brain. Our findings suggest a route of tryptophan metabolism in the lung and brain alternate to the common pathway leading to 5-hydroxyindoleacetic acid via 5-HT. This route is of physiologic and pathologic significance as many metabolites are pharmacologically active.

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Authors and Affiliations

  1. Department of Structural and Functional Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive West, 53706, Madison, WI, USA

    I. M. Keith & M. S. Brownfield

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  1. I. M. Keith
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  2. M. S. Brownfield
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Supported by the College of Agriculture and Life Sciences, University of Wisconsin-Madison and the Council for Tobacco Research USA, Inc. Grant #1437

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Keith, I.M., Brownfield, M.S. Evidence for presence of kynurenine in lung and brain of neonate hamsters. Histochemistry 83, 465–471 (1985). https://doi.org/10.1007/BF00509210

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