Zusammenfassung
Ein homogener und ein heterogener voltammetrischer Immunoassay (VIA) wurde mit Hilfe der Differential-Pulspolarographie untersucht durch Messung des Stromes des komplexierten Cobalts und der Brdicka-Stufe bei dem entsprechenden Potential als Funktion der immunchemischen Reaktion. Mit dem homogenen VIA zeigten Konzentrationen von 2,0×10−6 M von menschlichem Albumin und 1,1×10−6M von Ziegenantiserum gegen menschliches Albumin noch erkennbare Stromänderungen. Mit dem heterogenen VIA (bei dem Globulinproteine durch Ausfällung entfernt wurden) konnten noch Konzentrationen von 1,0×10−7 nachgewiesen werden.
Summary
Homogeneous and heterogeneous voltammetric immunoassay (VIA) of human albumin and goat antiserum to human albumin were studied by monitoring the current of complexed cobalt and the Brdicka wave at the appropriate potentials as a function of immunochemical reaction using differential pulse polarography. Using homogeneous VIA, concentration levels as low as 2.0×10−6 M human albumin and 1.1×10−6M goat antiserum to human albumin showed detectable change in the observed currents. With heterogeneous VIA, in which globulin proteins were removed by precipitation, levels of human albumin as low as 1.0×10−7M were detected.
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Alam, I.A., Christian, G.D. Voltammetric immunoassay of human albumin and goat antiserum to human albumin by cobalt labelling. Z. Anal. Chem. 318, 33–36 (1984). https://doi.org/10.1007/BF00532835
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DOI: https://doi.org/10.1007/BF00532835