Summary
S. typhimurium mutants with temperature-sensitive synthesis of DNA have been isolated. One of these mutants,dna-26, has been studied in detail. DNA synthesis is stopped indna-26 without any residual replication after shift to 42° though increase in cell mass is not inhibited. Mutantdna-26 shows increased sensitivity to deoxycholate, to nalidixic acid and rifampicin. This suggests a cell envelope defect. Inhibition of DNA synthesis at 42° can be phenotypically cured indna-26 by 0.25 M NaCl and KCl and 0.44 M sucrose but not by 0.44 M glycerol. This DNA synthesis induced by hypertonic medium proceeds at a slower rate than increase in cell mass but is predominantly due to normal sequential chromosome replication. The position of mutationdna-26 has been approximately mapped in thepurD region of the chromosome.
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Cieśla, Z., Bagdasarian, M. Impaired DNA synthesis and envelope defect in a mutant ofSalmonella typhimurium . Molec. gen. Genet. 116, 126–138 (1972). https://doi.org/10.1007/BF00582222
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DOI: https://doi.org/10.1007/BF00582222