Summary
During the summers of 1984 and 1985, adult red-spotted newts,Notophthalmus viridescens, were maintained in the laboratory at 23°±0.5°C under natural photoperiods. From each of the experimental animals, the right forelimb was amputated just proximal to the elbow. Control newts were not manipulated surgically. Eight, 15, and 22 days after the time of amputation, equal numbers of regenerating and control animals were sacrificed, and blood smears of each individual were prepared with Wright's stain.
Mean differential counts of leukocytes of the two groups of newts indicated that the relative number of neutrophils increased and the relative number of lymphocytes decreased in the regenerating animals as compared to their controls (Fig. 1 and Fig. 2). Earlier studies had shown that lymphopenia and neutrophilia occur in red-spotted newts treated with hydrocortisone or with ACTH or subjected to environmental stress (Bennett and Daigle 1983). Consequently, it is suggested that amputation and/or early regeneration may stimulate the increased production of hormones associated with stress in vertebrates, which may, in turn, influence regeneration, itself, and that the detailed study of the distribution of leukocytes inNotophthalmus viridescens may provide an assay with which to study the regulation of regeneration in this species.
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Bennett MF, Daigle KR (1983) Temperature, stress and the distribution of leukocytes in red-spotted newts,Notophthalmus viridescens. J Comp Physiol 153:81–83
Bennett MF, Gaudio CA, Johnson AO, Spisso JH (1972) Changes in the blood of newts,Notophthalmus viridescens, following the administration of hydrocortisone. J Comp Physiol 80:233–237
Bennett MF, Johnson AO (1973) Osmotic stress, ACTH and the white blood cell picture in newts,Notophthalmus viridescens. J Comp Physiol 82:333–338
Bennett MF, Neville CG (1975) Effects of cold shock on the distribution of leukocytes in goldfish,Carassius auratus. J Comp Physiol 98:213–216
Bennett MF, Reap LE (1978) Photoperiod, stress and the distribution of leukocytes in the peripheral blood ofNotophthalmus viridescens. J Comp Physiol 125:205–207
Dalton AJ, Selye H (1938) The blood picture and the blood sugar changes during the alarm reaction. Anat Rec 72 [Suppl]: 48–49
Liversage RA, McLaughlin DS, McLaughlin HMG (1985) The hormonal milieu in amphibian appendage regeneration. In: Sicard RE (ed) Regulation of vertebrate limb regeneration. Oxford University Press, New York Oxford, pp 54–80
Maier CE, Singer M (1977) The effect of light on forelimb regeneration in the newt. J Exp Zool 202:241–244
Rose P, Bennett MF (1978) Temperature and the distribution of leukocytes in newts,Notophthalmus viridescens. Am Zool 18:592
Schotté OE (1961) Systemic factors in initiation of regenerative processes in limbs of larval and adult amphibians. In: Rudnick D (ed) Synthesis of molecular and cellular structures. Ronald Press, New York, pp 161–192
Schotté OE, Sicard RE (1982) Cyclophosphamide-induced leukopenia and suppression of limb regeneration in the adult newt,Notophthalmus viridescens. J Exp Zool 222:199–202
Selye H (1950) The physiology and pathology of exposure to stress. Acta Inc, Montreal
Sicard RE (ed) (1985a) Regulation of vertebrate limb regeneration. Oxford University Press, New York Oxford
Sicard RE (1985b) Leukocytic and immunological influence on regeneration of amphibian forelimbs. In: Sicard RE (ed) Regulation of vertebrate limb regeneration. Oxford University Press, New York Oxford, pp 128–145
Wallace H (1981) Vertebrate limb regeneration. John Wiley and Sons, Chichester New York Brisbane Toronto
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Bennett, M.F. Stress and changes in the blood of newts,Notophthalmus viridescens, during early regeneration. J. Comp. Physiol. 159, 823–826 (1986). https://doi.org/10.1007/BF00603735
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DOI: https://doi.org/10.1007/BF00603735