Summary
Tomato plastid DNA has been isolated from leaf chloroplasts andPst1 fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largestPst1 fragment were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was generated by digestion of the clonedPst1 fragments and by hybridisation to Southern blots. The plastid DNA is a circular molecule of 156.6–159.4 kbp with a large inverted repeat, and shows high conservation of restriction sites with plastid DNA from tobacco andPetunia.
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References
Birnboim HC, Doty J (1979) Nucleic Acids Res 7:1513–1523
Bovenberg WA, Kool AJ, Nijkamp HJJ (1981) Nucleic Acids Res 9:503–517
Bowman CM, Dyer TA (1982) Anal Biochem 122:108–118
Burns DM, Beacham IR (1983) Anal Biochem 135:48–51
Clewell DB, Helinski DR (1969) Proc Natl Acad Sci USA 62: 1159–1166
Darby LA, Ritchie DB, Taylor IB (1978) Report of the Glass-house Crops Research Institute, pp 168–184
Davies JW, Cocking EC (1965) Planta 67:242–253
de Heij HT, Moeskops D-JM, Bovenberg WA, Bisanz C, Groot GSP (1983) Curr Genet 7:1–6
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Fluhr R, Edelman M (1981) Nucleic Acids Res 9:6841–6853
Fluhr R, Fromm H, Edelman M (1983) Gene 25:271–280
Grunstein M, Hogness DS (1975) Proc Natl Acad Sci USA 72: 3961–396
Hanahan D (1983) J Mol Biol 166:557–580
Harris WM (1970) Bot Gaz 131:163–166
Herrmann RG (1982) In: Edelman M et al (eds) Methods in chloplast molecular biology. Elsevier Biomedical Press, Amsterdam, pp 259–279
Iwatsuki N, Hirai A, Asahi T (1985) Plant Cell Physiol 26:599–601
Kushwaha SC, Suzue G, Subbarayan C, Porter JW (1970) J Biol Chem 245:4708–4717
Palmer JD (1982) Nucleic Acids Res 10:1593–1605
Palmer JD (1983) Nature (London) 301:92–93
Palmer JD, Thompson WF (1982) Cell 29:537–550
Palmer JD, Zamir D (1982) Proc Natl Acad Sci USA 79:5006–5010
Phillips AL (1985) Curr Genet 10:153–161
Rattanapanone N, Grierson D, Stein M (1977) Phytochemistry 16:629–633
Rattanapanone N, Speirs J, Grierson D (1978) Phytochemistry 17:1485–1486
Rosso SW (1968) J Ultrastruct Res 25:307–322
Schiller B, Herrmann RG, Melchers G (1982) Mot Gen Gent 186:453–459
Seyer P, Kowallik KV, Herrmann RG (1981) Curr Genet 3:189–204
Southern E (1979) Methods Enzymol 68:152–176
Vieira J, Messing J (1982) Gene 19:259–268
Wahl GM, Stern M, Stark GR (1979) Proc Natl Acad Sci USA 76:3683–3687
Zhu YS, Duvall EJ, Lovett PS, Kung SD (1982) Mol Gen Genet 187:61–66
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Phillips, A.L. Restriction map and clone bank of tomato plastid DNA. Curr Genet 10, 147–152 (1985). https://doi.org/10.1007/BF00636480
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DOI: https://doi.org/10.1007/BF00636480