Summary
FifteenRana pipiens were trained on a treadmill thrice weekly for 6.5 weeks to assess the effects of training on an animal that supports activity primarily through anaerobiosis. Endurance for activity increased 35% in these frogs as a result of training (P=0.006, Fig. 1). This increased performance was not due to enhanced anaerobiosis. Total lactate produced during exercise did not differ significantly for the trained or untrained animals in either gastrocnemius muscle (2.77±0.21 and 2.82±0.13 mg/g, respectively) or whole body (1.32±0.10 and 1.47±0.06 mg/g, respectively). Glycogen depletion also did not differ between the two groups (Fig. 2c). The primary response to training appeared to involve augmentation of aerobic metabolism, a response similar to that reported for mammals. Gastrocnemius muscles of trained frogs underwent a 38% increase over those of untrained individuals in the maximum activity of citrate synthase (14.5±1.0 and 10.5±0.9 μmoles/(g wet wt·min);P=0.008). This enzyme was also positively correlated with the level of maximum performance for all animals tested (r=0.61,P<0.01) and with the degree of improvement in the trained animals (r=0.72,P<0.05). In addition to an increased aerobic capacity, the trained animals demonstrated a greater removal of lactate from the muscle 15 min after fatigue (Fig. 2b).
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References
Alp, P.R., Newsholme, E.A., Zammit, V.A.: Activities of citrate synthase and NAD+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates. Biochem. J.154, 689–700 (1976)
Andersen, P., Henriksson, J.: Capillary supply of the quadriceps femoris muscle of man: adaptive response to exercise. J. Physiol.270, 677–690 (1977)
Baldwin, K.M., Klinkerfuss, G.H., Terjung, R.L., Molé, P.A., Holloszy, J.O.: Respiratory capacity of white, red and intermediate muscle: adaptive response to exercise. Am. J. Physiol.222, 373–378 (1972)
Bendall, J.R., Taylor, A.A.: The Meyerhof quotient and the synthesis of glycogen from lactate in frog and rabbit muscle. A reinvestigation. Biochem. J.118, 887–893 (1970)
Bennett, A.F., Gleeson, T.T.: Activity metabolism in the lizardSceloporus occidentalis. Physiol. Zool.49, 65–79 (1976)
Bennett, A.F., Licht, P.: Anaerobic metabolism during activity in lizards. J. Comp. Physiol.81, 277–288 (1972)
Bennett, A.F., Licht, P.: Anaerobic metabolism during activity in amphibians. Comp. Biochem. Physiol.48A, 319–327 (1974)
Brace, R.A.: Fitting straight lines to experimental data. Am. J. Physiol.233, R94-R99 (1977)
Carey, C.: Thermal physiology and energetics of boreal toads,Bufo boreas boreas. Ph.D. Dissertation, University of Michigan (1976)
Costill, D.L., Daniels, J., Evans, W., Fink, W., Krahenbuhl, G., Saltin, B.: Skeletal muscle enzymes and fiber composition in male and female track athletes. J. Appl. Physiol.40, 149–154 (1976)
Crabtree, B., Higgins, S.J., Newsholme, E.A.: The activities of pyruvate carboxylase, phosphoenolpyruvate carboxylase and fructose diphosphatase in muscles from vertebrates and invertebrates. Biochem. J.130, 391–396 (1972)
Dohm, G.L., Huston, R.L., Askew, E.W., Fleshood, H.L.: Effects of exercise, training and diet on muscle citric acid cycle enzyme activity. Can. J. Biochem.51, 849–854 (1973)
Gleeson, T.T.: The effects of training and captivity on the metabolic capacity of the lizardSceloporus occidentalis. J. Comp. Physiol.129, 123–128 (1979)
Gollnick, P.D., King, D.W.: Effect of exercise and training on mitochondria of rat skeletal muscle. Am. J. Physiol.216, 1502–1509 (1969)
Fitts, R.H., Booth, F.W., Winder, W.W., Holloszy, J.O.: Skeletal muscle respiratory capacity, endurance, and glycogen utilization. Am. J. Physiol.228, 1029–1033 (1975)
Hammond, B.R., Hickman, C.P.: The effect of physical conditioning on the metabolism of lactate, phosphate, and glucose in rainbow trout,Salmo gairdneri. J. Fish. Res. Bd. Canada23, 65–83 (1966)
Henriksson, J., Reitman, J.S.: Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activity and inactivity. Acta Physiol. Scand.99, 91–97 (1977)
Hermansen, L.: Lactate production during exercise. In: Muscle metabolism during exercise. Pernow, B., Saltin, B. (eds.), pp. 401–407. New York: Plenum 1971
Hermansen, L., Vaage, O.: Lactate disappearance and glycogen synthesis in human muscle after maximal exercise. Am. J. Physiol.233, E422-E429 (1977)
Holloszy, J.O.: Biochemical adaptations in muscle. Effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle. J. Biol. Chem.242, 2278–2282 (1967)
Holloszy, J.O., Booth, F.W.: Biochemical adaptations to endurance exercise in muscle. Ann. Rev. Physiol.38, 273–291 (1976)
Holloszy, J.O., Oscai, L.B., Don, I.J., Molé, P.A.: Mitochondrial citric acid cycle and related enzymes: adaptive response to exercise. Biochem. Biophys. Res. Commun.40, 1368–1373 (1970)
Hutchison, V.H., Turney, L.D.: Glucose and lactate concentrations during activity in the leopard frog,Rana pipiens. J. Comp. Physiol.99, 287–295 (1975)
Keppler, D., Decker, K.: Glycogen determination with amyloglucosidase. In: Methods of enzymatic analysis. Bergmeyer, H.U., (ed.), pp. 1127–1131. New York: Academic Press 1974
Krebs, H.A., Woodford, M.: Fructose 1,6-diphosphatase in striated muscle. Biochem. J.94, 436–445 (1965)
Maxwell, L.C., Barclay, J.K., Mohrman, D.E., Faulkner, J.A.: Physiological characteristics of skeletal muscles of dogs and cats. Am. J. Physiol.233, C14-C18 (1977)
Molé, P.A., Oscai, L.B., Holloszy, J.O.: Adaptation of muscle to exercise: increase in levels of palmityl CoA synthetase, carnitine palmityltransferase, and palmityl CoA dehydrogenase, and in the capacity to oxidize fatty acids. J. Clin. Invest.50, 2323–2330 (1971)
Opie, L.H., Newsholme, E.A.: The activities of fructose 1,6-diphosphatase, phosphofructokinase, phosphoenolpyruvate carboxykinase in white and red muscle. Biochem. J.103, 391–399 (1967)
Putnam, R.W.: Relationship between fatigue and changes of lactate and glycogen in muscles of anuran amphibians. Am. Zool.17, 893 (1977)
Srere, P.A.: Citrate synthase. In: Methods in enzymology, Vol. 13. Lowenstein, J.W. (ed.), pp. 3–11. New York: Academic Press 1969
Winder, W.W., Baldwin, K.M., Holloszy, J.O.: Enzymes involved in ketone utilization in different types of muscle: adaptation to exercise. Eur. J. Biochem.47, 461–467 (1974)
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Cummings, J.W. Physiological and biochemical adaptations to training inRana pipiens . J Comp Physiol B 134, 345–350 (1979). https://doi.org/10.1007/BF00710002
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DOI: https://doi.org/10.1007/BF00710002