Abstract
Quaking aspen (Populus tremuloides Michx.) staminate flower buds and the extended catkins are primary food resources for ruffed grouse (Bonasa umbellus). Winter feeding observations indicate that ruffed grouse select specific trees or clones of quaking aspen to feed in. Flower buds and catkins of quaking aspen were analyzed for secondary compounds (tannins, alkaloids, and phenolics) that might cause ruffed grouse to avoid trees with high levels of these compounds. Coniferyl benzoate, a compound that has not been previously found in quaking aspen, exists in significantly higher concentrations in buds from trees with no feeding history as compared to ruffed grouse feeding trees. Aspen catkins were also significantly lower in coniferyl benzoate than buds from the same tree. Ruffed grouse feeding preference was not related to the tannin or total phenolic levels found in buds or catkins. Buds from feeding trees had higher protein levels than trees with no feeding history; however, catkins did not differ from buds in protein concentration. The high use of extended catkins in the spring by ruffed grouse is probably due to a lower percentage of bud scale material in the catkin as opposed to the dormant bud. Bud scales contain almost all of the nontannin phenolics in catkins and dormant buds. A feeding strategy where bud scales are avoided may exist for other bird species that feed on quaking aspen. Dormant flower buds are significantly lower in protein-precipitable tannins than catkins and differ in secondary metabolite composition from other aspen foliage.
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References
Association of Official Agricultural Chemists. 1965. Official Methods of Analysis, 10th ed. A.O.A.C., Washington, D.C.
Barnes, B.V. 1966. The clonal growth habit of American aspens.Ecology 47:439–447.
Batzli, G.O. 1983. Responses of arctic rodent populations to nutritional factors.Oikos 40:396–406.
Bent, C.E. 1968. Life histories of North American cardinals, grosbeaks, buntings, towhees, finches, sparrows and allies.U.S. Natl. Mus. Bull. 237(pt. 1):1–602.
Bieleski, R.L. 1964. The problem of halting enzyme action when extracting plant tissues.Anal. Biochem. 9:431–442.
Blake, G.M. 1963. Clone identification and delineation in the aspens, PhD thesis. University of Minnesota, St. Paul.
Bradstreet, R.B. 1965. The Kjeldahl Method for Organic Nitrogen. Academic Press, New York.
Brown, C.P. 1946. Food of Maine ruffed grouse by season and cover types.J. Wildl. Manage. 10:17–28.
Bryant, J.P. 1981. The regulation of snowshoe hare feeding behavior during winter by plant antiherbivore chemistry, pp. 720–731,in K. Myers and C.D. MacInnes (eds.). Proceedings, First World Lagomorph Conference Guelph University, Guelph, Canada.
Bryant, J.P., andKuropat, P.J. 1980. Selection of winter forage by subarctic browsing vertebrates: The role of plant chemistry.Annu. Rev. Ecol. Syst. 11:261–285.
Bryant, J.P., Wieland, G.D., Reichardt, P.B., Lewis, V.E., andMcCarthy, M.C. 1983. Pinosylvin methyl ether deters snowshoe hare feeding on green alder.Science 222:1023–1025.
Bump, G.,Darrow, R.W.,Edminster, F.C., andCrissey, W.F. 1947. The Ruffed Grouse: Life History, Propagation, Management. New York Conservation Department.
Doerr, P.D., Keith, L.B., Rusch, D.H., andFischer, C.A. 1974. Characteristics of winter feeding aggregations of ruffed grouse in Alberta.J. Wildl. Manage. 38:601–615.
Egger, K. 1969. Plant phenol derivatives, pp. 686–705,in E. Stahl (ed.). Thin-Layer Chroma-tography. Springer-Verlag, Berlin.
Fried, B., andSherma, J. 1982. Thin-Layer Chromatography: Techniques and Applications. Marcel Dekker, New York.
Gullion, G.W. 1964. A discussion of ruffed grouse management problems in northern Minnesota. University of Minnesota Forest Wildlife Project Summary (unpublished).
Gullion, G.W. 1966. A viewpoint concerning the significance of studies of game bird food habits.Condor 68:372–376.
Gullion, G.W. 1970. Factors influencing raffed grouse populations.Trans. North Am. Wildl. Nat. Resour. Conf. 35:93–105.
Gullion, G.W. 1977. Forest manipulation for ruffed grouse.Trans. North Am. Wildl. Nat. Resour. Conf. 42:449–458.
Gullion, G.W. 1984. Grouse of the North Shore. Willow Creek Press, Oshkosh, Wisconsin.
Gullion, G.W. 1985. Aspen management—an opportunity for maximum integration of wood fiber and wildlife benefits.Trans. North Am. Wildl. Nat. Resour. Conf. 50:249–261.
Hagerman, A.E. 1987. Radial diffusion method for determining tannin in plant extracts.J. Chem. Ecol. 13:437–449.
Hagerman, A.E., andRobbins, C.T. 1987. Implications of soluble tannin-protein complexes for tannin analysis and plant defense mechanisms.J. Chem. Ecol. 13:1243–1257.
Hjorth, N. 1961. Eczematous allergy to balsams, allied perfumes and flavoring agents.Acta Dermato-Venereol. Suppl. 46:1–216.
Huempfner, R.A. 1981. Winter arboreal feeding behavior of ruffed grouse in east-central Minnesota. MS thesis. University of Minnesota, Minneapolis.
Huff, D.E. 1970. A study of selected nutrients in browse available to the ruffed grouse, MS thesis. University of Minnesota, St. Paul.
Jung, H.G., andFahey, G.C., Jr. 1983. Effects of phenolic monomers on rat performance and metabolism.J. Nutr. 113:546–556.
Kato, S. 1984. Process to formulate hypoallergenic jasmine oil.Perfum. Flavor. 9:137–140, 143–145.
Keith, L.B. 1963. Wildlife's Ten-Year Cycle. University of Wisconsin Press, Madison.
Lauckhart, J.B. 1957. Animal cycles and food.J. Wildl. Manage. 21:230–234.
Lea, A.G.H. 1978. The phenolics of ciders: Oligomeric and polymeric procyanidins.J. Sci. Food Agric. 29:471–477.
Lindroth, R.L., Scriber, J.M., andHsia, M.T.S. 1986. Differential responses of tiger swallowtail subspecies to secondary metabolites from tulip tree and quaking aspen.Oecologia (Berlin) 70:13–19.
Lindroth, R.L., Scriber, J.M., andHsia, M.T.S. 1987. Characterization of phenolic glycosides from quaking aspen.Biochem. Syst. Ecol. 15:677–680.
Martin, J.S., andMartin, M.M. 1982. Tannin assays in ecological studies: Lack of correlation between phenols, proanthocyanidins and protein-precipitating constituents in mature foliage of six oak species.Oecologia (Berlin) 54:205–211.
Mattes, B.R., Clausen, T.P., andReichardt, P.B. 1987. Volatile constituents of balsam poplar (Populus balsamifera L.): The phenolic glycoside connection.Phytochemistry 26:1361–1366.
Ott, L. 1984. An Introduction to Statistical Methods and Data Analysis. Duxbury Press, Boston.
Pearl, I.A., andDarling, S.F. 1959a. Studies on the barks of the family Salicaceae I. Tremuloiden a new glucoside from the bark ofPopulus tremuloides.J. Org. Chem. 24:731–735.
Pearl, I.A., andDarling, S.F. 1959b. Studies on the bark for the family Salicaceae II. Salreposide from the bark ofPopulus tremuloides.J. Org. Chem. 24:1616.
Pearl, I.A., andDarling, S.F. 1964. Further studies on the isolation of glucosides from the barks and leaves ofPopulus tremuloides andPopulus grandidentata.Tappi 47:377–380.
Pearl, I.A., andDarling, S.F. 1965. Benzoates of salicyloyl salicin from the barks and leaves ofPopulus grandidentata andPopulus tremuloides.Tappi 48:506–508.
Pearl, I.A., andDarling, S.F. 1967a. Studies on the leaves of the family Salicaceae VIII. Further studies on the leaves of triploidPopulus tremuloides.Tappi 50:193–195.
Pearl, I.A., andDarling, S.F. 1967b. Studies on the bark of the family Salicaceae XIV. Further studies on the bark of triploidPopulus tremuloides.Tappi 50:324–329.
Pearl, I.A., andDarling, S.F. 1971. Hot water phenolic extractives of the bark and leaves of diploidPopulus tremuloides.Phytochemistry 10:483–484.
Pearl, I.A., Darling, S.F., Dettaas, H., Loving, B.A., Scott, D.A., Turley, R.H., andWerth, R.E. 1961. Studies on the bark of the family Salicaceae.Tappi 44:475–478.
Pease, J.L., Vowles, R.H., andKeith, L.B. 1979. Interaction of snowshoe hares and woody vegetation.J. Wildl. Manage. 43:43–60.
Price, M.L., andButler, L.G. 1977. Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain.J. Agric. Food Chem. 25:1268–1273.
Reichardt, P.B., Bryant, J.P., Clausen, T.P., andWieland, G.D. 1984. Defense of winter-dormant Alaska paper birch against snowshoe hares.Oecologia (Berlin) 65:58–69.
Rhoades, D.F. 1983. Herbivore population dynamics and plant chemistry, pp. 155–220,in R.F. Denno and M.S. McClure (eds.). Variable Plants and Herbivores in Natural and Managed Systems. Academic Press, New York.
Rhoades, D.F. 1985. Offensive-defensive interactions between herbivores and plants: Their relevance in herbivore population dynamics and ecological theory.Am. Nat. 125:205–238.
Robbins, C.T., Mole, S., Hagerman, A.E., andHanley, T.A. 1987. Role of tannins in defending plants against ruminants: Reduction in dry matter digestion.Ecology 68:1606–1615.
Robinson, T. 1980. The Organic Constituents of Higher Plants. 4th ed. Cordus Press, Amherst, Massachusetts.
Rogler, J.C., Ganduglia, H.R.R., andElkin, R.G. 1985. Effect of nitrogen source level on the performance of chicks and rats fed low and high tannin sorghum.Nutr. Res. 5:1143–1151.
Rostagno, H.W., Featherston, W.R., andRogler, J.C. 1973. Studies on the nutritional value of sorghum grain with varying tannin content for chicks. 1. Growth studies.Poultry Sci. 52:765–772.
Santavy, F. 1969. Alkaloids, pp. 421–470,in E. Stahl (ed.). Thin-Layer Chromatography. Springer-Verlag, Berlin.
Schemnitz, S.D. 1970. Fall and winter feeding activities and behavior of ruffed grouse in Maine.Proc. Northeast. Sec. Wildl. Soc. Annu. Conf. 27:127–140.
Servello, F.A., andKirkpatrick, R.L. 1987. Regional variation in the nutritional ecology of ruffed grouse.J. Wildl. Manage. 51:749–770.
Shaub, M.S. 1956. Effect of native foods on evening grosbeak incursions.Bull. Mass. Audubon Soc. 40:481–488.
Silverstein, R.M., Keith, G.C., andMorrill, T.C. 1974. Spectrometric Identification of Organic Compounds. John Wiley & Sons, New York.
Stoll, R.J., Jr., McClain, M.W., Nixon, C.M., andWorley, D.M. 1980. Foods of ruffed grouse in Ohio. Ohio Fish and Wildlife Report 7, Ohio Dept. of Nat. Resources, Columbus.
Stollberg, B.P., andHine, R.L. 1952. Food habits studies of ruffed grouse, pheasant, quail and mink in Wisconsin. Wisconsin Conservation Department Technical Wildlife Bulletin 4, Wisconsin Conservation Dept., Madison.
Sunarjo, P.I. 1985. Phytochemical studies of quaking aspen (Populus tremuloides) and tulip tree (Liriodendron tulipifera) leaves and metabolism of precocene II in rats via the mercapturic acid pathway, PhD thesis. University of Wisconsin, Madison.
Svoboda, F.J., andGullion, G.W. 1972. Preferential use of aspen by ruffed grouse in northern Minnesota.J. Wildl. Manage. 36:1166–1180.
Swain, T. 1979. Tannin and lignins, pp. 657–682,in G.A. Rosenthal and D.H. Janzen (eds.). Herbivores: Their Interaction with Secondary Plant Metabolites. Academic Press, New York.
Takai, S., andHubbes, M. 1973. Polyphenoloxidase activity and growth inhibition ofHypoxylon pruinatum (Klotzsche) Cke. by aspen bark meal.Phytopath. Z. 78:97–108.
Thieme, H. 1965. Die Phenolglykoside der Salicacean. Untersuchungen über der jahrezeitlich bedingten Veränderugen der Glykosidkonzentrationen, über die Abhängigkeit des Glykosidgehatls von der Tageszeit und vom Alter der Pflanzenorgane.Pharmazie 20:688–671.
Thieme, H. 1967. Über die Phenolglykoside der Gattung Populus.Planta Med. 15:35–40.
Thieme, H., andBenecke, R. 1970. Die Phenolglykoside der Salicacean. Über die Glykosidfuhrung einheimischer bzw. in Mitteleuropa kultirerter Populus-Arten.Pharmazie 25:780–788.
Thieme, H., andBenecke, R. 1971. Die phenolglykosides der Salicacean. Untersuchungen über die glykosidakumulation in einigen Milleleuropaische Populus-arten.Pharmazie 26:227–231.
Togano, S.,Uehara, K.,Hayashi, K.,Nakamura, S., andOhta, S. 1983. Newly isolated and identified constituents of jasmine absolute. Paper presented at 9th International Congress of Essential Oils, Singapore: March 13–17, 1983.
Touchstone, J.C., andDobbins, M.F. 1978. Practice of Thin-Layer Chromatography. John Wiley & Sons, New York.
Vanderschaegen, P.V. 1970. Food habits of ruffed grouse at the Cloquet Forest Research Center, Minnesota. MS thesis. University of Minnesota, St. Paul.
Vohra, P., Kratzer, F.H., andJoslyn, M.A. 1966. The growth depressing and toxic effects of tannins to chicks.Poultry Sci. 45:135–142.
Watterson, J.J., andButler, L.G. 1983. Occurrence of an unusual leucoanthocyanidin and absence of proanthocyanidins in sorghum leaves.J. Agric. Food Chem. 31:41–45.
Wollenweber, E. 1975. Flavonoidmuster als systematisches Merkmal in der Gattung Populus.Biochem. Syst. Ecol. 3:35–45.
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Minnesota Agriculture Experiment Station Journal Series No. 16,953.
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Jakubas, W.J., Gullion, G.W. & Clausen, T.P. Ruffed grouse feeding behavior and its relationship to secondary metabolites of quaking aspen flower buds. J Chem Ecol 15, 1899–1917 (1989). https://doi.org/10.1007/BF01012275
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DOI: https://doi.org/10.1007/BF01012275