Summary
The cells ofCitrobacter intermedius (containing L-tyrosine phenol lyase which catalyses the synthesis of 3-fluoro-L-tyrosine fromo-fluorophenol, pyruvate and ammonia) were entrapped into 10% poly(vinyl alcohol) cryogel beads. Cryogenic immobilization resulted in a moderate increase of the synthetic activity of the cells. During continuous operation for one month in a packed bed column reactor the productivity of the immobilized biocatalyst decreased by 30%.
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Brodelius, P., and Vandamme, E. F. (1987). In: Biotechnology. A Comprehensive Treatise in 8 volumes., H.-J. Rehm and G. Reed eds., Weinheim: VCH Verlag, v. 7a, pp. 405–464.
Chibata, I., Tosa, T., and Kakimoto, T. (1986). In: Biotechnology of Amino Acids Production., K. Aida et al. eds., Amsterdam: Elsevier Sci. Publ., pp. 224–234.
Chibata, I., Tosa, T., and Sato, T. (1987). In: Biotechnology. A Comprehensive Treatise in 8 volumes., H.-J. Rehm and G. Reed eds., Weinheim: VCH Verlag, v. 7a, pp. 653–684.
Christopherson, R. I. (1985). Arch. Biochem. Biophys.240, 646–654.
Domotenko, L.V., Lozinsky, V.I., Vainerman, E.S., and Rogozhin, S.V. (1988). Vysokomolekul. soed.30A, 1661–1666.
Europ. Pat. (1983) No. 60052.
Faleev, N.G., Sadovnikova, M.S., Martinkova, N.S., and Belikov, V.M. (1981). Enzyme Microb. Technol.3, 219–224.
Ghosh, R., Bachofen, R., and Hauser, H. (1985). FEBS Lett.188, 107–111.
Hasimoto, S., Furukawa, K., and Hama, H. (1986). J. Jap. Sewage Wks. Assoc.23, 16–22.
Kukharchik, M.M., and Baramboim, N.K. (1972). Vysokomolekul. soed.12B, 843–846.
Lozinsky, V.I., Vainerman, E.S., Domotenko, L.V., Mamtsis, A.M., Titova, E.F., Belavtseva, E.M., and Rogozhin, S.V. (1986). Colloid & Polymer Sci.264, 19–24.
Manolov, R.G., Lozinsky, V. I., Tavobilov, I.M., Vainerman, E.S., Bezborodova, S.I., Rogozhin, S.V., and Bezborodov, A.M. (1987). Biotechnology and Bioindustry2, 3–5.
Manolov, R.G., Tavobilov, I.M., Lozinsky, V.I., Vainerman, E.S., Titova, E.F., Belavtseva, E.M., Bezborodova, S.I., Rogozhin, S.V., and Bezborodov, A.M. (1988). Prikl. biokhim. mikrobiol.24, 514–519.
Nagasawa, T., Utawaga, T., Goto, J., Zin, C.J., Tani, Y., Kumagai, H., and Yamada, H. (1981). Europ. J. Biochem.117, 33–40.
Reznikova, M.B., Novikova, M.A., and Zhdanov, G.L. (1965). Dokl. Akad. nauk SSSR161, 125–127.
USSR Pat. (1986). No. 1351971.
Watase, M., Nishinari, K., and Nambu, M. (1983). Cryo-Letters4, 197–200.
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Lozinsky, V.I., Faleev, N.G., Zubov, A.L. et al. Use of PVA-cryogel entrappedCitrobacter intermedius cells for continuous production of 3-fluoro-L-tyrosine. Biotechnol Lett 11, 43–48 (1989). https://doi.org/10.1007/BF01026784
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DOI: https://doi.org/10.1007/BF01026784