Summary
An alkalophilicStreptomyces which produced xylanase, isolated from soil, grew in a temperature range of 15–37°C. The pH optimum for growth was 10 and no growth occurred at pH 7. On a simple wheat bran medium the microorganism exhibited maximum enzyme secretion of 12 U/ml at pH 10. The enzyme had a broad pH optimum of 4.8–10 and the optimum temperature of 50°C. It was completely inactivated at 60°C in 2 h. The enzyme hydrolyzed xylan to a mixture of oligomeric products indicating that the main activity was of the endoxylanase type. The culture filtrate had no cellulase activity.
Similar content being viewed by others
References
Berghem, L. E. R. and Pettersson, L. G. (1973)Eur. J. Biochem. 37, 21–30.
Biely, P. (1985)Trends in Biotechnol. 3, 286–290.
Crawford, D. L. (1981)Biotechnol. Bioeng. Symp. 11, 275–291.
Davis, B. J. (1964)Ann. N.Y. Acad. Sci. 121 404–427.
Dekker, R. F. H. and Richard, J. N.Advances in Carbohydrate Chemistry, Academic Press, London (1976), pp. 278–352.
Honda, H., Kudo, T., Ikura, Y. and Horikoshi, K. (1985)Can. J. Microbiol. 31, 538–542.
Ishaque, M. and Klupfel, F. (1980)Can. J. Microbiol. 26, 183–189.
Klupfel, D., Shareack, F., Mondou, F. and Morosoli, R. (1986)Appl. Microbiol. Biotechnol. 24, 230–234.
Mandels, M. and Weber, J. (1969)Adv. Chem. Ser. 95, 395.
McCarthy, A. J., Peace, E. and Broda, P. (1985)Appl. Microbiol. Biotechnol. 21, 238–244.
Miller, G. L. (1959)Anal. Chem. 31, 426–428.
Okazaki, W., Akiba, T., Horikoshi, K. and Akahoshi, R. (1984)Appl. Microbiol. Biotechnol. 19, 335–340.
Reisfeld, R. A., Lewis, U. J. and Williams, D. E. (1962)Nature 195, 281–283.
Trevelyn, W. E., Procter, D. P. and Harrison, J. S. (1950)Nature, 166, 444–445.
Van Zyl, W. H. (1985)Biotechnol. Bioeng. 27, 1367–1373.
Wong, K. K. Y., Tan, L. U. L. and Saddler, J. N. (1988)Microbiol. Reviews, 52, 305–317.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vyas, P., Chauthaiwale, V., Phadatare, S. et al. Studies on the alkalophilicStreptomyces with extracellular xylanolytic activity. Biotechnol Lett 12, 225–228 (1990). https://doi.org/10.1007/BF01026804
Issue Date:
DOI: https://doi.org/10.1007/BF01026804