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Biotransformations by microbial Baeyer-Villiger monooxygenases stereoselective lactone formationin vitro by coupled enzyme systems

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Summary

The regio- and stereoselective biotransformation of bicyclo (3. 2. 0) hept-2-en-6-one by the NADH-dependent Baeyer-Villiger monooxygenase from camphorgrownPseudomonas putida NCIMB 10007 has been shown to yield a chiral lactone not accessible by curently-used biocatalysts. The biotransformation can be conductedin vitro using two alternative coupled enzyme systems (alcohol dehydrogenase and monooxygenase: formate dehydrogenase and monooxygenase) within situ recycling of NAD+/NADH.

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References

  • Alphand, V., Archelas, A. and Furstoss, R. (1989). Tet. Lett.30, 3663–3664.

    Google Scholar 

  • Alphand, V., Archelas, A. and Furstoss, R. (1990). Biocatalysis3, 73–83.

    Google Scholar 

  • Alphand, V. and Furstoss, R. (1992). J. Org. Chem.57, 1306–1309.

    Google Scholar 

  • Carnell, A.J., Roberts, S.M., Sik, V. and Willetts, A.J. (1990). J. Chem. Soc. Chem. Commun., 1438–1439.

  • Carnell, A.J., Roberts, S.M., Sik, V. and Willetts, A.J. (1991). J. Chem. Soc. Perkin Trans. I, 2385–2389.

  • Carnell, A. and Willetts, A. (1992). Biotechnol. Lett.14, 17–21.

    Google Scholar 

  • Griffin, M. and Trudgill, P.W. (1972). Biochem. J.129, 595–603.

    Google Scholar 

  • Jones, J.B. (1986). Tetrahedron43, 3351–3403.

    Google Scholar 

  • Levitt, M.S., Newton, R.F., Roberts, S.M. and Willetts, A.J. (1990). J. Chem. Soc. Chem. Commun., 619–620.

  • Ougham, H., Taylor, D. and Trudgill, P.W. (1983). J. Bact.153, 140–152.

    Google Scholar 

  • Rebolledo, F., Roberts, S. and Willetts, A. (1991). Biotechnol. Lett.13, 245–248.

    Google Scholar 

  • Sandey, H. (1991). Ph.D., University of Exeter.

  • Sandey, H. and Willetts, A. (1992). Biocatalysis-in press.

  • Taschner, M. and Chen, Q-Z. (1991). Bioorg. Med. Chem. Lett.1, 535–538.

    Google Scholar 

  • Taylor, D. and Trudgill, P.W. (1986). J. Bact.165, 489–497.

    Google Scholar 

  • Walsh, C.T. and Chen, J. (1988). Angew. Chem. Int. Ed. Engl.27, 333–343.

    Google Scholar 

  • Willetts, A.J., Knowles, C.J., Levitt, M.S., Roberts, S.M., Sandey, H. and Shipston, N.F. (1991). J. Chem. Soc. Perkin Trans. I, 1608–1610.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Exeter, EX4 4QG, Exeter, Devon, England

    Gideon Grogan & Andrew Willetts

  2. Department of Chemistry, University of Exeter, EX4 4QG, Exeter, Devon, England

    Stanley Roberts

Authors
  1. Gideon Grogan
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  2. Stanley Roberts
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  3. Andrew Willetts
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Grogan, G., Roberts, S. & Willetts, A. Biotransformations by microbial Baeyer-Villiger monooxygenases stereoselective lactone formationin vitro by coupled enzyme systems. Biotechnol Lett 14, 1125–1130 (1992). https://doi.org/10.1007/BF01027014

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