Summary
The morphological features and cellular relationships of neurotensin-containing axon terminals were studied at light and electron microscopic levels in the cat stellate ganglion using peroxidase and immunogold immunocytochemistry. By light microscopy, neurotensin immunoreactivity was detected within thin varicose fibres distributed throughout the ganglion. Immunoreactive fibres were no longer apparent following chronic deafferentation of the ganglion indicating that they were of extrinsic origin. Ultrastructural analysis of peroxidase immunostained material confirmed the presence of neurotensin immunoreactivity within a subpopulation of axonal varicosities which made synaptic contacts with the dendrites of ganglion cells. Within labelled varicosities neurotensin immunoreactivity was found by both immunoperoxidase and immunogold methods to be concentrated within large dense core vesicles 80–120 nm in diameter. These large dense core vesicles were characteristically distant from the active zone, in keeping with a possible extrasynaptic release of the peptide.
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Bachoo, M., Ciriello, J. &Polosa, C. (1987) Effect of preganglionic stimulation on neuropeptide-like immunoreactivity in the stellate ganglion of the cat.Brain Research 400, 377–82.
Bachoo, M. &Polosa, C. (1988) Cardioacceleration produced by close intra-arterial injection of neurotensin into the stellate ganglion of the cat.Canadian Journal of Physiology and Pharmacology 66, 408–12.
Bayer, V. E., Towle, A. C. &Pickel, V. M. (1991) Vesicular and cytoplasmic localization of neurotensin-like immunoreactivity (NTLI) in neurons postsynaptic to terminals containing NTLI and/or tyrosine hydroxylase in the rat central nucleus of the amygdala.Journal of Neuroscience Research 30, 398–413.
Carraway, R. &Leeman, S. E. (1973) The isolation of a new hypotensive peptide, neurotensin, from bovine hypothalamus.Journal of Biological Chemistry 248, 6854–61.
Carraway, R. &Leeman, S. E. (1975) The aminoacid sequence of hypothalamic peptide neurotensin.Journal of Biological Chemistry 250, 1907–11.
Caverson, M. M., Bachoo, M., Ciriello, J. &Polosa, C. (1989) Effect of preganglionic stimulation or chronic decentralization on neurotensin-like immunoreactivity in sympathetic ganglia of the cat.Brain Research 482, 365–70.
De MeY, J. (1983) A critical review of light and electron microscopic immunocytochemical techniques used in neurobiology.Journal of Neuroscience Methods 7, 1–18.
Forehand, C. J. (1987) Ultrastructural analysis of the distribution of synaptic boutons from labeled preganglionic axons on rabbit ciliary neurons.Journal of Neuroscience 7, 3274–81.
Heym, C., Reineck, M., Weihe, E. &Forssmann, W. G. (1984) Dopamine-beta-hydroxylase, neurotensin, substance P, vasoactive intestinal polypeptide and enkephalin immunohistochemistry of paravertebral and prevertebral ganglia in the cat.Cell and Tissue Research 235, 411–18.
Kenigsberg, R. L., Semenenko, F. M. &Cuello, A. C. (1990) Development of a bi-specific monoclonal antibody for simultaneous detection of rabbit IgG and horseradish peroxidase: use as a general reagent in immunohistochemistry and enzyme-linked immunoabsorbent assay.Journal of Histochemistry and Cytochemistry 38, 191–8.
Krukoff, T. L., Ciriello, J. &Calaresu, F. R. (1985) Segmental distribution of peptide-like immunoreactivity in cell bodies of the thoracolumbar sympathetic nuclei of the cat.Journal of Comparative Neurology 240, 90–102.
Lundberg, J. M., Rokaeus, A., Hökfelt, T., Rosell, S., Brown, M. &Goldstein, M. (1982) Neurotensin-like immunoreactivity in the preganglionic sympathetic nerves and in the adrenal medulla of the cat.Acta Physiologica Scandinavica 114, 153–5.
Maher, E., Bachoo, M., Cernacek, P. &Polosa, C. (1991) Dynamics of neurotensin stores in the stellate ganglion of the cat.Brain Research 562, 258–64.
Maher, E., Bachoo, M. &Polosa, C. (1992) The role of action potentials in the homeostasis of neurotensin 1–13 stores in preganglionic axon terminals of the cat stellate ganglion.Physiology Canada 23, 185 (abstract)
Morales, M. A., Bachoo, M., Collier, B., Beaudet, A. &Polosa, C. (1992) Ultrastructural localization of neurotensin (NT) immunoreactivity (IR) in the stellate ganglion (SG) of the cat.Society for Neuroscience Abstracts 18, 266.
Nishi, S. &Katayama, Y. (1980) The non-cholinergic excitatory transmission in sympathetic ganglia. InAdvances in Physiological Sciences, Vol 4: Physiology of Excitable Membranes (edited bySalanki, J.) pp. 323–7. New York: Pergamon Press.
NjÅ, A. &Purves, D. (1977) Specific innervation of guineapig superior cervical ganglion cells by preganglionic fibres arising from different levels of the spinal cord.Journal of Physiology 264, 565–83.
Novikoff, A. B., Novikoff, P. M., Quintana, N. &Davis, C. (1972) Diffusion artifacts in 3,3′-diaminobenzidine cytochemistry.Journal of Histochemistry and Cytochemistry 20, 745–9.
Oldfield, B. J. &McLachlan, E. M. (1978) Localization of sensory neurons traversing the stellate ganglion of the cat.Journal of Comparative Neurology 182, 915–22.
Pardini, B. J. &Wurster, R. D. (1984) Identification of the sympathetic preganglionic pathway to the cat stellate ganglion.Journal of the Autonomic Nervous System 11, 13–26.
Schultzberg, M. &Lindh, B. (1988) Transmitters and peptides in autonomic ganglia. InHandbook of Chemical Neuroanatomy, Vol. 6: The Peripheral Nervous System (edited byBjorklund, A., Hökfelt, T. &Owman, C.) pp. 297–326. Amsterdam: Elsevier.
Szulczyk, A. &Szulczyk, P. (1987) Spinal segmental preganglionic outflow to cervical sympathetic trunk and postganglionic cardiac sympathetic nerves.Brain Research 421, 127–34.
Weldon, P., Bachoo, M. &Polosa, C. (1990) Depletion by preganglionic stimulation and post-stimulus recovery of large dense core vesicles in synaptic boutons of the cat superior cervical ganglion.Brain Research 516, 341–4.
Williams, J. T., Katayama, Y. &North, R. A. (1979) The action of neurotensin on single myenteric neurons.European Journal of Pharmacology 59, 181–6.
Woulfe, J. &Beaudet, A. (1992) Neurotensin terminals form synapses primarily with neurons lacking detectable tyrosine hydroxylase immunoreactivity in the rat substantia nigra and ventral tegmental area.Journal of Comparative Neurology 321, 163–76.
Yaksh, R. L., Michener, S. R., Bailey, J. E., Marty, G. L., Lucas, D. L., Nelson, D. K., Roddy, D. R. &Go, V. L. W. (1988) Survey of distribution of substance P, vasoactive intestinal polypeptide, cholecystokinin, neurotensin, metenkephalin, bombesin and PHI in the spinal cord of cat, dog, sloth and monkey.Peptides 9, 357–72.
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Morales, M.A., Bachoo, M., Beaudet, A. et al. Ultrastructural localization of neurotensin immunoreactivity in the stellate ganglion of the cat. J Neurocytol 22, 1017–1021 (1993). https://doi.org/10.1007/BF01235745
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DOI: https://doi.org/10.1007/BF01235745