Skip to main content
Log in

The division of protoplasts fromAsparagus officinalis L. and their growth and differentiation

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Protoplasts have been isolated from cladodes ofAsparagus officinalis L., and cell wall regeneration and division observed. Callus derived from isolated protoplasts has been induced to differentiate roots and shoots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bayley, J. M., J. King, andO. L. Gamborg, 1972: The ability of amino compounds and conditioned medium to alleviate the reduced nitrogen requirement of soybean cells grown in suspension cultures. Planta (Berl.)105, 25–32.

    Google Scholar 

  • Carlson, P. S., H. H. Smith, andR. D. Dearing, 1972: Parasexual interspecific plant hybridization. Proc. nat. Acad. Sci. (U.S.A.)69, 2292–2294.

    Google Scholar 

  • Evans, P. K., A. G. Keates, andE. C. Cocking, 1972: Isolation of protoplasts from cereal leaves. Planta (Berl.)104, 178–181.

    Google Scholar 

  • Gamborg, O. L., 1970: The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol.45, 373–375.

    Google Scholar 

  • Halperin, W., andD. F. Wetherell, 1965: Ammonium requirements for Embryogenesisin vitro. Nature2, 519–520.

    Google Scholar 

  • Harada, H., K. Ohyama, andJ. Cheruel, 1972: Effects of coumarin and other factors on the modification of form and growth of isolated mesophyll cells. Z. Pflanzenphysiol.66, 307–324.

    Google Scholar 

  • —, 1973: A new method for obtaining protoplasts from mesophyll cells. Z. Pflanzenphysiol.69, 77–80.

    Google Scholar 

  • Harvey, R. G., T. J. Muzik, andR. L. Warner, 1973: Differences in nitrogen requirements of two clones ofConvolvulus arvensis in vitro. Amer. J. Bot.60, 76–79.

    Google Scholar 

  • Kameya, T., andJ. Uchimiya, 1972: Embryoids derived from isolated protoplasts of carrot. Planta (Berl.)103, 356–360.

    Google Scholar 

  • Mackenzie, I. A., D. Bui-Dang-Ha, and M. R.Davey, 1972: Some aspects of the isolation, fine structure and growth of protoplasts fromAsparagus officinalis L. Colloques internationaux C.N.R.S. No. 212-Protoplastes et fusion de cellules somatiques végétales, 291–299.

  • Nagata, T., andI. Takebe, 1971: Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta (Berl.)99, 12–20.

    Google Scholar 

  • — —, 1970: Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta (Berl.)92, 301–308.

    Google Scholar 

  • Nitsch, J. P., etK. Ohyama, 1971: Obtention de plantes à partir de protoplastes haploïdes cultivésin vitro. C. R. Acad. Sci. (Paris)273, 801–804.

    Google Scholar 

  • Ohyama, K., O. L. Gamborg, andR. A. Miller, 1972: Uptake of exogenous DNA by plant protoplasts. Canad. J. Bot.50, 2077–2080.

    Google Scholar 

  • Power, J. B., S. E. Cummins, andE. C. Cocking, 1970: Fusion of isolated plant protoplasts. Nature225, 1016–1018.

    Google Scholar 

  • Sakai, F., andI. Takebe, 1970: RNA and protein synthesis in protoplasts isolated from tobacco leaves. Biochem. Biophys. Acta224, 531–540.

    Google Scholar 

  • Steward, F. C., andM. O. Mapes, 1971: Morphogenesis and plant propagation in aseptic cultures ofAsparagus. Bot. Gaz.132, 70–79.

    Google Scholar 

  • Stuart, R., andH. E. Street, 1969: Studies on the growth in culture of plant cells. IV. The initiation of division in suspensions of stationary phase cells ofAcer pseudoplatanus L. J. Exp. Bot.20, 556–571.

    Google Scholar 

  • Wilmar, C., andM. Hellendoorn, 1968: Growth and morphogenesis ofAsparagus cells culturedin vitro. Nature217, 369–370.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bui-Dang-Ha, D., Mackenzie, I.A. The division of protoplasts fromAsparagus officinalis L. and their growth and differentiation. Protoplasma 78, 215–221 (1973). https://doi.org/10.1007/BF01275692

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01275692

Keywords

Navigation