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Immunogold localization of a developmentally regulated, tapetal-specific, 15 kDa lily anther protein

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Summary

We have confirmed that the LLA-15 polypeptide ofLilium longiflorum is (a) tapetum specific with some expression possible in the adjacent middle layer cells and (b) relatively abundant as evidenced by the high density of gold particles localized to the tapetal cells. We have established that the protein is cytoplasmic and not associated with organelles, membranes, extracellular matrix or wall. We also report an amino acid composition of the molecule and a partial sequence which bears no resemblance to any protein yet described.

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Abbreviations

BSA:

bovine serum albumin

PMSF:

phenyl methyl sulfonyl fluoride

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Author notes

  1. J. -L. Wang

    Present address: Department of Surgery, UCLA Medical Center, Los Angeles, California, USA

  2. C. -S. Wang

    Present address: Agricultural Biotechnology Laboratories, National Chung-Hsing University, Taichung, Taiwan

Authors and Affiliations

  1. Department of Botany and Plant Sciences, University of California, 92521, Riverside, CA, USA

    R. A. Balsamo, J. -L. Wang, K. J. Eckard, C. -S. Wang & E. M. Lord

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  1. R. A. Balsamo
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  2. J. -L. Wang
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  3. K. J. Eckard
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  4. C. -S. Wang
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  5. E. M. Lord
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Balsamo, R.A., Wang, J.L., Eckard, K.J. et al. Immunogold localization of a developmentally regulated, tapetal-specific, 15 kDa lily anther protein. Protoplasma 189, 17–25 (1995). https://doi.org/10.1007/BF01280288

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  • DOI: https://doi.org/10.1007/BF01280288

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