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Effect of glucose on jejunal water and solute absorption in the presence of glycodeoxycholate and oleate in man

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Abstract

Jejunal perfusion studies were performed in 12 healthy volunteers to study the effects of 14 and 56 mM glucose on fluid secretion induced by 5 mM glycodeoxycholate or 7 mM oleate. Glucose enhanced water absorption under control conditions and reduced water secretion induced by glycodeoxycholate or oleate (P<0.01). As has been observed previously, glycodeoxycholate and oleate inhibited glucose absorption (P<0.001) and significant linear relationships existed between net water movement and glucose absorption. Glycodeoxycholate also reduced the absorption of 14 mM arabinose (P<0.05) and oleate reduced the absorption of 56 mM mannitol (P<0.05). Reduced solute absorption in the presence of glycodeoxycholate and oleate, therefore, cannot be attributed to an effect on active transport alone. The relationships between sodium transport and water absorption varied with the glucose concentration in the perfusion solutions. Similarly, the relationships between glucose absorption and sodium absorption varied with glucose concentration. The data suggest that a significant amount of glucose can be absorbed without concomitant absorption of sodium. The data indicate that glucose absorption can stimulate water absorption directly without the mediation of sodium and that water movement follows glucose at a rate which maintains isotonicity.

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References

  1. Forth W, Rummel W, Glasner H: Zur resorptionshemmenden Wirkung von Gallensäuren. Naunyn-Schmiedebergs Arch Pharmak Exp Pathol 254: 364–380, 1966

    Google Scholar 

  2. Wingate DL, Phillips SF, Hofmann AF: Effect of glycineconjugated bile acids with and without lecithin on water and glucose absorption in perfused human jejunum. J Clin Invest 52: 1230–1236, 1973

    Google Scholar 

  3. Krag E, Phillips SF: Effect of free and conjugated bile acids on net water, electrolyte, and glucose movement in the perfused human ileum. J Lab Clin Med 83: 947–956, 1974

    Google Scholar 

  4. Mekhjian HS, Phillips SF, Hofmann AF: Colonic secretion of water and electrolytes induced by bile acids; perfusion studies in man. J Clin Invest 50: 1569–1577, 1971

    Google Scholar 

  5. Ammon HV, Phillips SF: Inhibiton of ileal water absorption by intraluminal fatty acids. Influence of chain length, hydroxylation, and conjugation of fatty acids. J Clin Invest 53: 205–210, 1974

    Google Scholar 

  6. Ammon HV, Thomas PJ, Phillips SF: Effects of long chain fatty acids on solute absorption: Perfusion studies in the human jejunum. Gut 18: 805–812, 1977

    Google Scholar 

  7. Wanitschke R, Ammon HV: Effects of dihydroxy bile acids and hydroxy fatty acids on the absorption of oleic acid in the human jejunum. J Clin Invest 61: 178–186, 1978

    Google Scholar 

  8. Chadwick VS, Phillips SF, Hofmann AF: Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). Gastroenterology 73: 247–251, 1977

    Google Scholar 

  9. Gaginella TS, Chadwick VS, Debongnie JC, Lewis JC, Phillips SF: Perfusion of rabbit colon with ricinoleic acid: Dose-related mucosal injury, fluid secretion, and increased permeability. Gastroenterology 73: 95–101, 1977

    Google Scholar 

  10. Dobbins JW, Binder HJ: Effect of bile salts and fatty acids on the colonic absorption of oxalate. Gastroenterology 70: 1096–1100, 1976

    Google Scholar 

  11. Small DM: A Classification of biologic lipids based upon their interaction in aqueous systems. J Am Oil Chem Soc 45: 108–119, 1968

    Google Scholar 

  12. Conley, DR, Coyne MJ, Bonorris GG, Chung A, Schoenfield LJ: Bile acid stimulation of colonic adenylate cyclase and secretion in the rabbit. Am J Dig Dis 21: 453–458, 1976

    Google Scholar 

  13. Binder HJ, Filburn C, Volpe BT: Bile salt alteration of colonic electrolyte transport: Role of cyclic adenosine monophosphate. Gastroenterology 68: 503–508, 1975

    Google Scholar 

  14. Racusen LC, Binder HJ: Ricinoleic acid stimulation of active anion secretion in colonic mucosa of the rat. J Clin Invest 63: 743–749, 1979

    Google Scholar 

  15. Nell G, Forth W, Freiberger T, Rummel W, Wanitschke R: Characterization of permeability changes by test molecules in rat colonic mucosa under the influence of sodium deoxycholate.In Advances in Bile Acid Research, S. Matern, J. Hackenschmidt, P. Back, W. Gerok (eds). New York, FK Schattauer, 1975, pp 491–424

    Google Scholar 

  16. Saunders DR, Hedges JR, Sillery J, Esther L, Matsumura K, Rubin CE: Morphological and functional effects of bile salts on rat colon. Gastroenterology 68: 1236–1245, 1975

    Google Scholar 

  17. Cline WS, Lorenzsonn V, Benz L, Bass P, Olsen WA: The effects of sodium ricinoleate on small intestinal function and structure. J Clin Invest 58: 380–390, 1976

    Google Scholar 

  18. Kimberg DV, Field M, Johnson J, Henderson A, Gershon E: Stimulation of intestinal mucosal adenyl cyclase by cholera enterotoxin and prostaglandins. J Clin Invest 50: 1218–1230, 1971

    Google Scholar 

  19. Carpenter CCJ, Sack RB, Feeley JC, Steenberg RW: Site and characteristics of electrolyte loss and effect of intraluminal glucose in experimental canine cholera. J Clin Invest 47: 1210–1220, 1968

    Google Scholar 

  20. Serebro HA, Bayless TM, Hendrix TR, Iber FL, McGonagle T: Absorption ofd-glucose by the rabbit jejunum during cholera toxin-induced diarrhea. Nature 217: 1272–1273, 1968

    Google Scholar 

  21. Hofmann AF: The function of bile salts in fat absorption: The solvent properties of dilute micellar solutions of conjugated bile salts. Biochem J 89: 57–68, 1963

    Google Scholar 

  22. Sladen GE, Dawson AM: Interrelationships between the absorptions of glucose, sodium and water by the normal human jejunum. Clin Sci 36: 119–132, 1969

    Google Scholar 

  23. Neale RJ, Wiseman G: The use of dietary-restricted rat intestine for active transport studies. J Physiol 205: 159–178, 1969

    Google Scholar 

  24. Wingate DL, Sandberg RJ, Phillips SF: A comparison of stable and14C-labelled polyethylene glycol as volume indicators in the human jejunum. Gut 13: 812–815, 1972

    Google Scholar 

  25. Roe JH, Rice EW: Photometric method for determination of free pentoses in animal tissues. J Biol Chem 173: 507–512, 1948

    Google Scholar 

  26. Sweeley CC, Bentley R, Makita M, Wells WW: Gas-liquid chromatography of trimethysily derivatives of sugars and related substances. J Am Chem Soc 85: 2497–2507, 1963

    Google Scholar 

  27. Talalay P: Enzymic analysis of steroid hormones.In Methods of Biochemical Analysis, D. Glick (ed). New York, Interscience Publishers, 1960, pp 119–143

    Google Scholar 

  28. Wingate DL, Krag E, Mekhjian HS, Phillips SF: Relationships between ion and water movement in the human jejunum, ileum and colon during perfusion with bile acids. Clin Sci Mol Med 45: 593–606, 1973

    Google Scholar 

  29. Zar JH: Biostatistical Analysis. Englewood Cliffs, New Jersey, Prentice-Hall, 1974

    Google Scholar 

  30. Harries JT, Sladen GE: The effects of different bile salts on the absorption of fluid, electrolytes, and monosaccharides in small intestine of the ratin vivo. Gut 13: 596–603, 1972

    Google Scholar 

  31. Crane RK: The gradient hypothesis and other models of carrier-mediated active transport. Rev Physiol Biochem Pharmacol 78: 99–159, 1977

    Google Scholar 

  32. Fordtran JS: Stimulation of active and passive sodium absorption by sugars in the human jejunum. J Clin Invest 55: 728–737, 1975

    Google Scholar 

  33. Olsen WA, Ingelfinger FJ: The role of sodium in intestinal glucose absorption in man. J Clin Invest 47: 1133–1142, 1968

    Google Scholar 

  34. Adibi SA: Leucine absorption rate and net movements of sodium and water in human jejunum. J Appl Physiol 28: 753–757, 1970

    Google Scholar 

  35. Nalin DR, Cash RA, Rahman M, Yunus MD: Effect of glycine and glucose on sodium and water absorption in patients with cholera. Gut 11: 768–772, 1970

    Google Scholar 

  36. Matuchansky C, Bernier JJ: Effect of prostaglandin E1 on glucose, water, and electrolyte absorption in the human jejunum. Gastroenterology 64: 1111–1118, 1973

    Google Scholar 

  37. Charney AN, Donowitz M: Functional signifance of Na+-K+-ATPase:In vivo ouabain inhibition. Am J Physiol 3: E629–636, 1978

    Google Scholar 

  38. Donowitz M, Charney AN, Heffernan JM: Effect of serotonin treatment on intestinal transport in the rabbit. Am J Physiol 1: E85–94, 1977

    Google Scholar 

  39. Binder HJ, Dobbins JW, Racusen LC, Whiting DS: Effect of propranolol on ricinoleic acid- and deoxycholic acid-induced changes of intestinal electrolyte movement and mucosal permeability. Gastroenterology 75: 668–673, 1978

    Google Scholar 

  40. Caspary WF: Inhibition of active hexose and amino acid transport by conjugated bile salts in rat ileum. Eur J Clin Invest 4: 17–24, 1974

    Google Scholar 

  41. Young TK, Lee SC: Effect of mannitol on sodium retention during whole-gut perfusion. Aust J. Exp Biol Med Sci 56: 609–615, 1978

    Google Scholar 

  42. Broor SL, Slota T, Ammon HV: Cholesterol reduces the effects of dihydroxy bile acids and fatty acids on water and solute transport in the human jejunum. J Clin Invest 65: 920–925, 1980

    Google Scholar 

  43. Lamabadusuriya SP, Guiraldes E, Harries JT: Influence of mixtures of taurocholate, fatty acids, and monoolein on the toxic effects of deoxycholate in rat jejunumin vivo. Gastroenterology 69: 463–469, 1975

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Veterans Administration Medical Center, Wood (Milwaukee), Wisconsin

    B. D. Brown MD & H. V. Ammon MD

  2. Department of Medicine, Gastroenterology Section/111C, The Medical College of Wisconsin, 53193, Milwaukee, Wisconsin

    B. D. Brown MD & H. V. Ammon MD

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  2. H. V. Ammon MD
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Brown, B.D., Ammon, H.V. Effect of glucose on jejunal water and solute absorption in the presence of glycodeoxycholate and oleate in man. Digest Dis Sci 26, 710–717 (1981). https://doi.org/10.1007/BF01316860

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  • DOI: https://doi.org/10.1007/BF01316860

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