Summary
The maturation process of Japanese encephalitis (JE) virus in C6/36 cells in vitro and in mouse brain cells in vivo was studied by electron microscopy. In the C6/36 cell infection, 500 to 2250 virions per cell were released into the medium during the period of study; yet, no virus budding process was observed at the host cell membranes. JE virions at various maturation stages appeared within the cisternae of rough endoplasmic reticulum (RER) of infected cells at 24 hours p.i.; and, although C6/36 cells did not show a well-developed Golgi apparatus, the virions appeared to be carried to the cell surface within host-cell secretory vesicles for extracellular release as early as 24 hours p.i. The occurrence of a secretory-type intracellular transport of maturing JE virus particles was well recognizable in brain cells of infected mice, in which JE virus particles were found almost exclusively in the cisternae of RER, in the Golgi apparatus, and in various vesicles, including coated vesicles, in the vicinity of the Golgi apparatus. Our previous study of dengue-2 virus morphogenesis and our present study of JE virus morphogenesis differed substantially at various stages of maturation. Possible mechanisms which explain these differences were discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asafo-Adjei E, Bernard EF, Hase T (1987) Growing and embedding monolayer cells in situ in Beem capsule caps for light and electron microscopy. J Electron Microsc Techn 5: 367–372
Eckels KH, Brandt WE, Harrison VR, McCown JM, Russell PK (1976) Isolation of a temperature-sensitive dengue-2 virus under conditions suitable for vaccine development. Infect Immun 14: 1221–1227
Farquhar MG, Palade GE (1981) The Golgi apparatus (complex)-(1954–1981)-from artifact to central stage. J Cell Biol 91 [3, Pt2]: 77 s-103 s
Filshie BK, Rehacek J (1968) Studies of the morphology of Murray Valley encephalitis and Japanese encephalitis viruses growing in cultured mosquito cells. Virology 34: 435–443
Griffiths G, Pfeiffer S, Simons K, Matlin K (1985) Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. J Cell Biol 101: 949–964
Griffiths G, Simons K (1986) The trans Golgi network: sorting at the exit site of the Golgi complex. Science 234: 438–443
Hase T, Summers PL, Eckels KH, Baze WB (1987) An electron and immunoelectron microscopic study of dengue-2 virus infection of cultured mosquito cells: maturation events. Arch Virol 92: 273–291
Leary K, Blair CD (1980) Sequential events in the morphogenesis of Japanese encephalitis virus. J Ultrastruct Res 72: 123–129
Matsumura T, Shiraki K, Sashikata T, Hotta S (1977) Morphogenesis of dengue-1 virus in cultures of a human leukemic leukocyte line (J-111). Microbiol Immunol 21: 329–334
Murphy FA, Harrison AK, Gary GW Jr, Whitfield SG, Forrester FT (1968) St. Louis encephalitis virus infection of mice. Electron microscopic studies of central nervous system. Lab Invest 19: 652–662
Ota Z (1965) Electron microscopic study of the development of Japanese B encephalitis virus in porcine kidney stable (PS) cells. Virology 25: 372–378
Sriurairatna S, Bhamarapravati N, Phalavadhtana O (1973) Dengue virus infection of mice: morphology and morphogenesis of dengue type-2 virus in suckling mouse neurons. Infect Immun 8: 1017–1028
Tooze J, Tooze SA (1986) Clathrin-coated vesicular transport of secretory proteins during the formation of ACTH-containing secretory granules in AtT 20 cells. J Cell Biol 103: 839–850
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hase, T., Summers, P.L., Eckels, K.H. et al. Maturation process of Japanese encephalitis virus in cultured mosquito cells in vitro and mouse brain cells in vivo. Archives of Virology 96, 135–151 (1987). https://doi.org/10.1007/BF01320956
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01320956