Summary
The complete nucleotide sequence of apple mosaic ilarvirus (ApMV) RNA-3 has been determined from cloned viral cDNAs. The 5′ terminus of RNA-3 was determined by direct RNA sequencing, while the 3′ end was determined by polyadenylation of genomic RNA and sub-cloning using oligo dT. ApMV RNA-3 is 2056 bases in length and encodes at least two open reading frames. It is similar in size and genome organization to the RNA-3 of other members of theBromoviridae, which includes ilarviruses. The CP gene is in the 3′ half of the molecule, and another large open reading frame is upstream of the CP gene and can potentially encode a protein of 32 400 daltons. This peptide is the same size and shows limited sequence homology to an open reading frame located at the 5′ end of RNA 3 in tobacco streak and prune dwarf ilarviruses and alfalfa mosaic virus, which is postulated to be the viral movement protein. The nucleic acid sequence was not homologous to tobacco streak virus, prune dwarf virus, alfalfa mosaic virus or other members of theBromoviridae. The 5′-non-coding region of ApMV RNA-3 contains a 15 base palindromic sequence which encloses a sequence resembling the ICR-2 regions of eukaryotic tRNA gene promoters.
Similar content being viewed by others
References
Alrefai RH, Shiel PJ, Domier LL, D'Arcy CJ, Berger PH, Korban SS (1994) The nucleotide sequence of apple mosaic virus coat protein gene has no homology with otherBromoviridae coat protein genes. J Gen Virol 75: 2847–2850
Bachman EJ, Scott SW, Xin G, Vance VB (1994) The complete nucleotide sequence of prune dwarf virus RNA 3. Virology 201: 127–131
Barker RF, Jarvis NP, Thompson DV, Loesch-Fries LS, Hall TC (1983) Complete nucleotide sequence of alfalfa mosaic virus RNA-3. Nucleic Acids Res 11: 2881–2891
Berg JM (1990) Zinc finger domains: hypotheses and current knowledge. Annu Rev Biophys Biophys Chem 19: 405–421
Cornelissen BJC, Janssen H, Zuidema D, Bol JF (1984) Complete nucleotide sequence of tobacco streak virus RNA3. Nucleic Acids Res 12: 2427–2437
Dasgupta R, Kaesberg P (1982) Complete nucleotide sequences of the coat protein messenger RNAs of brome mosaic virus and cowpea chlorotic mottle virus. Nucleic Acids Res 10: 703–713
Ge X, Scott SW (1994) The nucleotide sequence of citrus leaf rugose ilarvirus RNA-2. J Gen Virol 75: 2841–2856
Greber RS, Teakle DS, Mink GI (1992) Thrips-facilitated transmission of prune dwarf andPrunus necrotic ringspot viruses from cherry pollen to cucumber. Plant Dis 76: 1039–1041
Gonsalves D, Fulton RW (1977) Activation ofPrunus necrotic ringspot virus and rose mosaic virus by RNA 4 components of some ilarviruses. Virology 81: 398–407
Francki RIB, Fauquet CM, Knudson DL, Brown F (1991) Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York (Arch Virol [Suppl] 2)
Fulton RW (1967) Purification and serology of rose mosaic virus. Phytopathology 57: 1197–1201
Fulton RW, Hamilton RI (1960) Serological differentiation of prune dwarf and sour cherry necrotic ringspot viruses. Phytopathology 50: 635 (Abstr.)
Houser-Scott F, Baer ML, Liem KF, Cai JM, Gehrke L (1994) Nucleotide sequence and structural determinants of specific binding of coat protein or coat protein peptides to the 3′ untraslated region of alfalfa mosaic virus RNA 4. J Virol 68: 2194–2205
Koonin EV, Mushegian AR, Ryabov EV, Dolja V (1991) Diverse groups of plant RNA and DNA viruses share related movement proteins that may possess chaperone-like activity. J Gen Virol 72: 2895–2903
Mise K, Allison RF, Janda M, Ahlquist P (1993) Bromovirus movement protein genes play a crucial role in host specificity. J Virol 67: 2815–2823
Natsuaki T, Mayo MA, Jolly CA, Murant AF (1991) Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome. J Gen Virol 72: 2183–2189
Ong CA, Mink GI (1989) Evaluation of agarose gel-electrophoresis for resolving nucleoprotein components ofPrunus necrotic ringspot virus. Phytopathology 79: 613–619
Pogue GP, Marsh LE, Connell JP, Hall TC (1992) Requirement for ICR-like sequences in the replication of brome mosaic virus genomic RNA. Virology 188: 742–753
Sanchez-Navarro JA, Pallas V (1994) Nucleotide sequence of apple mosaic ilarvirus RNA 4. J Gen Virol 75: 1441–1445
Sehnke PC, Mason AM, Hood SJ, Lister RM, Johnson JE (1989) A “zinc-finger”-type binding domain in tobacco streak virus coat protein. Virology 168: 48–56
van der Vossen EAG, Neelemen L, Bol JF (1993) Role of the 5′ leader sequence of alfalfa mosaic virus RNA 3 in replication and translation of the viral RNA. Nucleic Acids Res 21: 1361–1367
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shiel, P.J., Alrefai, R.H., Domier, L.L. et al. The complete nucleotide sequence of apple mosaic virus RNA-3. Archives of Virology 140, 1247–1256 (1995). https://doi.org/10.1007/BF01322750
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01322750