Summary
Field trials carried out in 1965 and 1966 showed that 2,4-DB, alone or in combination with dalapon, reduced nodulation and tended to decrease the efficiency of nitrogen fixation in birdsfoot trefoil. Dalapon appeared to enhance the inhibitory action of 2,4-DB on nodulation. No obvious cytological differences could be detected in the nodules or in the isolated bacteroids of field-treated and untreated plants. Under growth chamber conditions, 2,4-DB drastically reduced trefoil growth and nodulation particularly in treatments where the herbicide came directly in contact with the plants. It appears that the reduction in nodulation and nitrogen fixation is a result of plant damage and abnormal root growth caused by 2,4-DB application.
Autoradiographs indicated that the translocation of the herbicide was rapid, with detectable concentrations observed in young leaves, leafveins, roots, and nodules 12 hours after leaf-feeding of 2,4-DB-1-C14. The radio-activity appeared to accumulate with time (up to 5 days) in the growing root tips and nodules. Fractionation of excised nodules from trefoil plants demonstrated the presence of radioactivity in the cell debris, bacteroids, 29,000g pellet, plant ribosomes, and the soluble portion. The greatest accumulation of radioactivity occurred in the soluble fraction.
The degradation of 2,4-DB and 2,4-D in trefoil was demonstrated by the evolution of C14O2 from non-nodulated and aseptically growing plants leaf-fed with 2,4-DB-1-C14 or 2,4-D-1-C14.
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References
Anderson, G. R. and Baker, G. O., Some effects of 2,4-D in representative Idaho soils. Agron. J.42, 456–458 (1950).
Bergersen, F. J. and Briggs, M. S., Studies on the bacterial component of soybean root nodules: Cytology and organization in the host tissue. J. Gen. Microbiol.19, 482–490 (1958).
Carlyle, R. E. and Thorpe, J. D., Some effects of ammonium and sodium 2,4-dichlorophenoxyacetates on legumes and theRhizobium bacteria. J. Amer. Soc. Agron.39, 929–936 (1947).
Crafts, A. S. and Yamaguchi, S., Autoradiographic method for studying absorption and translocation of herbicides using C14 labeled compounds. Hilgardia27, 421–454 (1958).
Crafts, A. S. and Yamaguchi, S., The autoradiography of plant materials. California Agr. Expt. Sta. Ext. Service Manual35. (1964).
Fertig, S. N., Loos, M. A., Gutenmann, W. H., and Lisk, D. J., Formation of 2,4-D in 4-(2,4-DB)-treated timothy, birdsfoot trefoil, and sterile pea plants. Weeds12, 147–148 (1964).
Fletcher, W. W., Dickenson, P. B., Forrest, J. D., and Raymond, J. C., The effect of soil-applications of certain substituted phenoxy-acetic acid and phenoxy-butyric acids on the growth and nodulation ofTrifolium repens sylvestre. Phyton9, 41–46 (1957).
Hoagland, D. R. and Arnon, D. I., The water-culture method for growing plants without soil. California Agr. Expt. Sta. Circ.347 (1950).
Jordan, D. C. and Garrard, E. H., Studies on the legume root nodule bacteria. I. Detection of effective and ineffective strains. Can. J. Botany29, 360–372 (1951).
Jordan, D. C. and Grinyer, I., Electron microscopy of the bacteroids and root nodules ofLupinus luteus. Can. J. Microbiol.11, 721–725 (1965).
Linscott, D. L., Degradation of 4-(2,4-dichlorophenoxy)-butyric acid (4-(2,4-DB)) in plants, Agr. Food Chem.12, 7–10 (1964).
Nilsson, P. E., The influence of antibiotics and antagonists on symbiotic nitrogen fixation of legume cultures. Kungl. Lantbrukshogsk. Ann.23, 219–253 (1957).
Norris, L. A. and Freed, V. H., The absorption, translocation and metabolism characteristics of 4-(2,4-dichlorophenoxy)butyric acid in Bigleaf maple. Weed Research6, 283–291 (1966).
Nutman, P. S., Physiological studies on nodule formation. I. The relation between nodulation and lateral root formation in red clover. Ann. Botany (Lond.)12, 81–96 (1948).
Payne, M. G. and Fults, J. L., Some effects of 2,4-D, DDT and Colorado 9 on root nodulation in the common bean. Agron. J.39, 52–55 (1947).
Redemann, C. T. and Meikle, R. W., The inhibition of several enzyme systems by 2,2-dichloropropionate. Arch. Biochem.59, 106–112 (1955).
Society of American Bacteriologists., Manual of microbiological methods. McGraw-Hill Book Company, Inc. New York. (1957).
Steel, R. G. D. and Torrie, J. H. Principles and Procedures of Statistics with Special Reference to the Biological Sciences. McGraw-Hill Book Co. Inc., New York. (1960).
van Schreven, D. A., Effects of added sugar and nitrogen on nodulation of legumes. Plant and Soil11, 93–112 (1959).
Virtanen, A. I., Erkana, J. and Linkola, H., On the relation between nitrogen fixation and leghemoglobin content of leguminous root nodules. II. Acta. Chem. Scand.1, 861–870 (1947).
Virtanen, A. I., Jorma, J., Linkola, H., and Linnasalmi, A., On the relation between nitrogen-fixation and leghemoglobin content of leguminous root nodules. I. Acta. Chem. Scand.1, 90–111 (1947).
Wilson, S. B., and Hallsworth, E. G., The distribution of cobalt inT. subterraneum Plant and Soil23, 60–78 (1965).
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4-(2,4-dichlorophenoxy) butyric acid.
2,2 dichloropropionic acid.
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Garcia, M.M., Jordan, D.C. Action of 2,4-DB and dalapon on the symbiotic properties ofLotus corniculatus (birdsfoot trefoil). Plant Soil 30, 317–334 (1969). https://doi.org/10.1007/BF01349519
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DOI: https://doi.org/10.1007/BF01349519