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Assay for adipose differentiation using primary culture of adipocyte precursors

  • Published:
Journal of tissue culture methods

Summary

This paper describes a method to establish primary and secondary cultures of adipocyte precursors isolated from inguinal fat pads of 48-hr-old rats in defined medium. The culture medium consists of DME-F12 medium supplemented with fibronectin, insulin, transferrin, and fibroblast growth factor. Data presented indicate that 90% of the cells plated in the 4F medium differentiate in 7 d. These cultures provide appropriate differentiation assay systems to characterize regulators of differentiation acting on normal cells derived from the adipose tissue.

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Authors and Affiliations

  1. W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, 12946, Lake Placid, NY

    Ginette Serrero & Dianne Mills

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  1. Ginette Serrero
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  2. Dianne Mills
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Serrero, G., Mills, D. Assay for adipose differentiation using primary culture of adipocyte precursors. Journal of Tissue Culture Methods 10, 75–81 (1986). https://doi.org/10.1007/BF01404597

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  • DOI: https://doi.org/10.1007/BF01404597

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