Abstract
A Chinese HCV cDNA clone, designated as Q379, 379 nucleotides in length, was selected by recombinant immunoscreening from a random-primed Chinese HCV λgt11 library. DNA sequencing demonstrated that Q379 situated from positions 7314 to 7695 at NS5 region corresponded with the American prototype HCV nucleotide sequence. The homologies of Q379 with the equivalent sequences of the prototype were found to be 72.8% in nucleotides and 73.8% in amino acids, respectively. Hydrophobicity profile shows that Q379-encoded polypeptide (126 amino acid residues) contains two hydrophilic areas. This Chinese HCV clone would be valuable in basic and clinical studies of Chinese HCV infection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Arima T, Nagashima H, Murakami S (1989) Cloning of a cDNA associated with acute and chronic hepatitis C infection generated from patients serum RNA. Gastroenterol Jpn 24:540–544
Choo QL, Richman KH, Han JH (1991) Genetic organization and diversity of the hepatitis C virus. Proc Natl Acad Sci USA 88:2451–2455
Kato N, Hijikata M, Ootsuyama Y (1991) Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc Natl Acad Sci USA 87:9524–9528
Qi Z, Pan W, Du P (1992) Detection of serum antibody to hepatitis C virus and amplification of its genomic cDNA. Acad J Sec Mil Med Univ 13:11–16
Qi Z, Pan W, Kong X (1992) Immunoscreening and identification of Chinese HCV genomic cDNA λgt11 library. Acad J Sec Mil Med Univ 13:401–410
Qi Z, Pan W, Du P (1992) Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus. Acad J Sec Mil Med Univ 13:301–306
Sambrook J (1989) Molecular cloning: screening expression libraries with antibodies and oligonucleotides. Book two, 2nd ed. New York: Cold Spring Harbor Laboratory Press 12.1–12.44
Takamizawa A, Mori C, Fuke I (1991) Structure and organization of the hepatitis C virus genome isolated from human carriers. J Virol 65:1105–1113
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Qi, Z., Arima, T. Clone Q379 immunoscreened from a Chinese HCV cDNA λgt11 library. Current Microbiology 28, 161–163 (1994). https://doi.org/10.1007/BF01571058
Issue Date:
DOI: https://doi.org/10.1007/BF01571058