Summary
The 5′-terminal half of RNA 1 and whole of RNA 2 of barley mild mosaic bymovirus Na1 strain (BaMMV-Na1) were sequenced to give together with the published data its complete genomic sequence. BaMMV-Na1 RNA 1 and RNA 2 consist of 7 263 and 3 516 nucleotides, excluding the 3′ poly A tails, respectively. RNA 1 encodes a single large polyprotein of 2 258 amino acids (Mr 256 K), containing eight putative functional proteins, and RNA 2 also encodes one polyprotein of 891 amino acids (Mr 98 K), containing two functional proteins. These functional proteins are arranged in the same manner as in RNA 1 and RNA 2 of barley yellow mosaic bymovirus (BaYMV), and significant amino acid sequence homology (25–58%) exists between the proteins of the two viruses. The BaMMV-Na1 proteins show less amino acid sequence homology (18–32%) with the corresponding proteins of potyviruses or rymoviruses than with those of Ba YMV. Comparisons of the BaMMV-Na1 proteins with the corresponding proteins of other partially sequenced BaMMV isolates show 87–98% amino acid sequence identity. There is 91–94% nucleotide sequence identity between the 3′ non-coding regions (NCRs) in RNA 1 or RNA 2 of BaMMV-Na1 and other BaMMV isolates, but only 68–72% identity between the 5′ NCRs in RNA 2 of BaMMV-Na1 and other BaMMV isolates.
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The nucleotide sequence data described in this paper will appear in the DDBJ, EMBL and GenBank nucleotide databases under the accession numbers D83408 (RNA 1) and D83409 (RNA 2).
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Kashiwazaki, S. The complete nucleotide sequence and genome organization of barley mild mosaic virus (Na1 strain). Archives of Virology 141, 2077–2089 (1996). https://doi.org/10.1007/BF01718216
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DOI: https://doi.org/10.1007/BF01718216