Summary
A new type of p-Benzoquinone activated support (agarose, polyvinyl alcohol, chitosan) which can be stored for more than one year, is described. Coupling yields of 10–95% were obtained for various compounds (propylamine, serum albumin, alpha-amylase). A retained activity of 150% for the immobilized laccase, was obtained. This peculiar result is probably due to the presence of hydroquinone groups on the support, which, as a substrate of laccase, can generate a simultancous affinity retention of the enzyme.
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Mateescu, MA., Weltrowska, G., Agostinelli, E. et al. Ready to use p-Benzoquinone activated supports for biochemical coupling, with special applications for laccase immobilization. Biotechnol Tech 3, 415–420 (1989). https://doi.org/10.1007/BF01875011
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DOI: https://doi.org/10.1007/BF01875011