Summary
Using the FPG staining technique we have confirmed that incubation temperature has a marked effect on the proliferation rate of cultured lymphocytes. With 48 h-cultures the proportion of 2nd-cycle cells increases linearly over the temperature range 36–39°C. However the absolute size of the increase exhibits interdonor variability.
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P. Perry and S. Wolff, Nature, Lond.251, 156 (1974).
P.E. Crossen and W.F. Morgan, Exp. Cell Res.104, 453 (1977).
R.J. Purrott, N. Vulpis and D.C. Lloyd, Mutation Res.69, 275 (1980).
A. Leonard and G. Decat, Can. J. Genet. Cytol.21, 473 (1979).
P.P.W. van Buul and A.T. Natarajan, Mutation Res.70, 61 (1980).
M.A. Bender and J.G. Brewen, Mutation Res.8, 383 (1969).
P.A. Borodkin, Radiobiology19, 150 (1979).
C.F. Salodino and H.A. Johnson, Exp. Cell Res.8, 248 (1974).
R.J. Purrott and D.C. Lloyd, The Study of Chromosome Aberration Yield in Human Lymphocytes as an Indicator of Radiation Dose, I. Techniques. National Radiological Protection Board Report No. R2, Harwell, Oxon, 1972.
D. Scott and C.Y. Lyons, Nature, Lond.278, 756 (1979).
N.O. Bianchi, M.S. Bianchi and M. Larramendy, Mutation Res.63, 317 (1979).
K. Wyszynska and J. Liniecki, Mutation Res.73, 101 (1980).
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We would like to acknowledge the assistance of the Commission of the European Communities for their provision of a travel grant.
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Purrott, R.J., Vulpis, N. & Lloyd, D.C. The influence of incubation temperature on the rate of human lymphocyte proliferation in vitro. Experientia 37, 407–408 (1981). https://doi.org/10.1007/BF01959890
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DOI: https://doi.org/10.1007/BF01959890