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Methods for isolation ofBordetella pertussis from patients with whooping cough

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Abstract

Culture remains the standard method for diagnosis of whooping cough. While in the past attempts at isolatingBordetella pertussis from patients with suspected whooping cough were often unsuccessful, new methods have recently been developed which are suitable for use in routine microbiology laboratories. Recent advances include the use of calcium alginate tipped swabs for taking nasopharyngeal swabs, use of charcoal horse blood agar for transport and culture, and the inclusion of cephalexin as a selective agent in agar media. Experience shows that careful application of these new methods often enablesBordetella pertussis to be isolated from clinical specimens, thereby permitting a diagnosis to be made at an early stage of the disease.

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References

  1. Onorato, I. M., Wassilak, S. G. F. Laboratory diagnosis of pertussis: the state of the art. Pediatric Infectious Disease Journal 1987, 6: 145–151.

    PubMed  Google Scholar 

  2. Brooks, A. M., Bradford, W. L., Berry, G. P. The method of nasopharyngeal culture in the diagnosis of whooping cough. Journal of the American Medical Association 1942, 120: 883–885.

    Google Scholar 

  3. Kwantes, W., Joynson, D. H. M., Williams, W. O. Bordetella pertussis isolation in general practice: 1977–79 whooping cough epidemic in West Glamorgan. Journal of Hygiene 1983, 90: 149–158.

    PubMed  Google Scholar 

  4. Preston, N. W. Valore della tecniche microbiologiche nella diagnosi e nella prevenzione della pertosse. Clinica e Laboratorio 1979, 3: 207–220.

    Google Scholar 

  5. Linnemann, C. C., Bass, J. W. Bordetella infections. In: Balows, A., Hausler, W. J. (ed.): Diagnostic procedures for bacterial, mycotic and parasitic infections. American Public Health Association, Washington, DC, 1981, p. 249–260.

    Google Scholar 

  6. Marcon, M. J., Hamoudi, A. C., Cannon, H. J., Hribar, M. M. Comparison of throat and nasopharyngeal swab specimens for culture diagnosis ofBordetella pertussis infection. Journal of Clinical Microbiology 1987, 24: 1109–1110.

    Google Scholar 

  7. Lautrop, H. Laboratory diagnosis of whooping cough orBordetella infections. Bulletin of the World Health Organization 1960, 23: 15–31.

    Google Scholar 

  8. Parker, C. D., Payne, B. J. Bordetella. In: Lennette, E. H., Balows, A., Hausler, W. J., Shadomy, H. J. (ed.): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1985, p. 394–399.

    Google Scholar 

  9. Abbott, J. D., Macaulay, M. E., Preston, N. W. Bacteriological diagnosis of whooping cough. Broadsheet 105. Association of Clinical Pathologists, London, 1982.

    Google Scholar 

  10. Wort, A. J. Bacteriological diagnosis of pertussis. Lancet 1983, i: 766.

    Google Scholar 

  11. Ross, P. W., Cumming, C. G. Isolation ofBordetella pertussis from swabs. British Medical Journal 1981, 283: 403–404.

    PubMed  Google Scholar 

  12. Hoppe, J. E., Weiß, A. Recovery ofBordetella pertussis from four kinds of swabs. European Journal of Clinical Microbiology 1987, 6: 203–205.

    PubMed  Google Scholar 

  13. Hoppe, J. E., Wörz, S., Botzenhart, K. Comparison of specimen transport systems forBordetella pertussis. European Journal of Clinical Microbiology 1986, 5: 671–673.

    PubMed  Google Scholar 

  14. Antonis, A. H. Isolation ofBordetella pertussis from pernasal swabs stored in Stuart's transport medium. Journal of Medical Microbiology 1970, 3: 184–186.

    PubMed  Google Scholar 

  15. Hunter, P. R. Survival ofBordetella pertussis in transport media. Journal of Clinical Pathology 1986, 39: 119–120.

    PubMed  Google Scholar 

  16. Appelbaum, P. C., Spangler, S. K., Crist, R., Crist, A. E. Survival of bacteria in Difco Culture Swab and Marion Culturette II transport systems. Journal of Clinical Microbiology 1988, 26: 136–138.

    PubMed  Google Scholar 

  17. Regan, J., Lowe, F. Enrichment medium for the isolation ofBordetella. Journal of Clinical Microbiology 1977, 6: 303–309.

    PubMed  Google Scholar 

  18. Jones, G. L., Kendrick, P. L. Study of a blood-free medium for transport and growth ofBordetella pertussis. Health Laboratory Science 1969, 6: 40–45.

    PubMed  Google Scholar 

  19. Gilligan, P. H., Fisher, M. C. Importance of culture in laboratory diagnosis ofBordetella pertussis infections. Journal of Clinical Microbiology 1984, 20: 891–893.

    PubMed  Google Scholar 

  20. Preston, N. W. Technical problems in the laboratory diagnosis and prevention of whooping cough. Laboratory Practice 1970, 19: 482–486.

    PubMed  Google Scholar 

  21. Sutcliffe, E. M., Abbott, J. D. Selective medium for the isolation ofBordetella pertussis andparapertussis. Journal of Clinical Pathology 1972, 25: 732–733.

    PubMed  Google Scholar 

  22. Hoppe, J. E., Vogl, R. Comparison of three media for culture ofBordetella pertussis. European Journal of Clinical Microbiology 1986, 5: 361–362.

    PubMed  Google Scholar 

  23. Ahmad, F., Calder, M. A. Isolation ofBordetella pertussis: benefits of using both Bordet-Gengou and charcoal agar media. Journal of Clinical Pathology 1984, 37: 1071–1072.

    PubMed  Google Scholar 

  24. Rowatt, E. The growth ofBordetella pertussis: a review. Journal of General Microbiology 1957, 17: 297–326.

    PubMed  Google Scholar 

  25. Aoyama, T., Murase, Y., Iwata, T., Imaizumi, A., Suzuki, Y., Sato, Y. Comparison of blood-free medium (cyclodextrin solid medium) with Bordet-Gengou medium for clinical isolation ofBordetella pertussis. Journal of Clinical Microbiology 1986, 23: 1046–1048.

    PubMed  Google Scholar 

  26. Stainer, D. W., Scholte, M. J. A simple chemically defined medium for the production of phase IBordetella pertussis. Journal of General Microbiology 1971, 63: 211–220.

    Google Scholar 

  27. Hoppe, J. E., Haug, A., Botzenhart, K. Susceptibility ofBordetella pertussis andBordetella parapertussis to twenty-four antibiotics. Chemotherapy 1987, 33: 250–254.

    PubMed  Google Scholar 

  28. Stauffer, L. R., Brown, D. R., Sandstrom, R. E. Cephalexin-supplemented Jones-Kendrick charcoal agar for selective isolation ofBordetella pertussis: comparison with previously described media. Journal of Clinical Microbiology 1983, 17: 60–62.

    PubMed  Google Scholar 

  29. Bannatyne, R. M., Cheung, R. Susceptibility ofBordetella pertussis to cephalosporin derivatives and imipenem. Antimicrobial Agents and Chemotherapy 1984, 26: 604–605.

    PubMed  Google Scholar 

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  1. University Children's Hospital, Rümelinstr. 23, D-7400, Tübingen, FRG

    J. E. Hoppe

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Hoppe, J.E. Methods for isolation ofBordetella pertussis from patients with whooping cough. Eur. J. Clin. Microbiol. Infect. Dis. 7, 616–620 (1988). https://doi.org/10.1007/BF01964238

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