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Phythämagglutinin (PHA) und Serumproteine in der Lymphocytenkultur

I. Nachweis von fördernden und hemmenden Serumfaktoren

Phytohemagglutinin (PHA) and serum proteins in lymphocyte culture

I. Incidence for growth promoting and inhibiting serum factors

  • Published:
Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

The influence of serum fractions on PHA stimulated lymphocytes has been determined by mRNA synthesis. Serum without lipoproteins was fractionated by Sephadex G 200 chromatography. Fraction I had a stimulating effect in low concentrations and an inhibiting one in high doses. The growth promoting effect of Fraction I was due to α2-macroglobulin. This was indicated by purified α2-macroglobulin and α2-macroglobulin deficient serum. Fraction II had a strong inhibiting effect, whereas Fraction III contained a growth promoting protein. The growth potential was markedly reduced in α1-antitrypsin deficient serum. For this reason the stimulating effect of Fraction III may be caused by α1-antitrypsin.

Zusammenfassung

Die Wirkung von Serumfraktionen auf das Wachstum PHA-stimulierter Lymphocyten wurde auf Grund ihres RNS-Stoffwechsels beurteilt. Die Chromatographie von lipoproteinfreiem Serum über Sephadex G 200 ergab drei Serumfraktionen. Fraktion I stimulierte in geringen Konzentrationen und hatte in hohen Konzentrationen einen hemmenden Effekt. Durch weitere Fraktionierungen und durch die Verwendung von Mangelserum konnte die wachstumsfördernde Wirkung auf α2-Makroglobulin zurückgeführt werden. Einen hemmenden Effekt zeigte die Serumfraktion II, während Fraktion III deutlich stimulierend wirkte. Mit Hilfe eines Mangelserums konnte der wachstumsfördernde Effekt von Fraktion III auf α1-Antitrypsin zurückgeführt werden.

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  1. Medizinische Universitätsklinik Marburg a. d. Lahn, Deutschland

    K. Havemann, H. M. Dosch & S. Bürger

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  1. K. Havemann
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  2. H. M. Dosch
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  3. S. Bürger
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Havemann, K., Dosch, H.M. & Bürger, S. Phythämagglutinin (PHA) und Serumproteine in der Lymphocytenkultur. Z. Gesamte Exp. Med. 153, 297–307 (1970). https://doi.org/10.1007/BF02048091

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