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Growth regulation of rabbit gastric epithelial cells and protooncogene expression

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Abstract

We recently developed a primary culture system for gastric epithelial cells from adult rabbits that allows the investigation of growth regulation at the cellular level. In this study, we demonstrated that epidermal growth factor (EGF), insulin, and dibutyryl adenosine 3′,5′-cyclic monophosphate (dBcAMP) all stimulated cell proliferation. Insulin and dB-cAMP potentiated the stimulation of cell proliferation by EGF, while transforming growth factor-β1 (TGF-β1) inhibited it. Expression of c-fos and c-myc was induced in response to the stimulation by these growth regulators, but the degree of expression did not necessarily correlate with the effects of these agents on cell proliferation. In conclusion, EGF, insulin, and dBcAMP were positive growth regulators, while TGF-β1 was a negative regulator in gastric epithelial cells. These growth modulators may exert their effects by distinct pathways from a standpoint of the expression of c-fos and c-myc.

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Authors and Affiliations

  1. From the 2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan

    Kenta Yoshiura MD, Shinichi Ota MD, PhD, Akira Terano MD, PhD, Morio Takahashi MD, Yasuo Hata MD, Takao Kawabe MD, Hiroyuki Mutoh MD, PhD, Hideyuki Hiraishi MD, PhD, Ryo Nakata MD, Kenichi Okano MD, PhD & Masao Omata MD, PhD

  2. the Institute for Adult Diseases Asahi Life Foundation, Tokyo, Japan

    Kenta Yoshiura MD, Shinichi Ota MD, PhD, Akira Terano MD, PhD, Morio Takahashi MD, Yasuo Hata MD, Takao Kawabe MD, Hiroyuki Mutoh MD, PhD, Hideyuki Hiraishi MD, PhD, Ryo Nakata MD, Kenichi Okano MD, PhD & Masao Omata MD, PhD

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  2. Shinichi Ota MD, PhD
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  3. Akira Terano MD, PhD
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Yoshiura, K., Ota, S., Terano, A. et al. Growth regulation of rabbit gastric epithelial cells and protooncogene expression. Digest Dis Sci 39, 1454–1463 (1994). https://doi.org/10.1007/BF02088048

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  • DOI: https://doi.org/10.1007/BF02088048

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