Abstract
A lymphotropic papovavirus was isolated from a lymphoblastoid cell line of African green monkey (AGM) cells which also contained a herpesvirus and a paramyxovirus-like agent. The papovavirus was analyzed by restriction endonuclease cleavage; its biochemical and serological crossreactivity with SV40 and host range have been determined. Thus far, only B-lymphoblasts of primate and human origin have been found to be susceptible to infection. Although more than 50% of the tested monkey sera were reactive with antigens of this virus, all human sera tested failed to react. Cleavage patterns and hybridization studies with the viral DNA indicate that the virus represents a novel member of the papovavirus group that is characterized by its lymphotropic host range. Papovavirus particles were also demonstrated in a human lymphoblastoid cell line (CCRF-SB) originally derived from a leukemic child. These cells revealed nuclear fluorescence when tested with human sera, but failed to react with AGM sera. Although characterization of this agent has not yet been completed, available evidence suggests that it represents another lymphotropic papovavirus which seems to be spread within the human population.
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References
Carroll, R.B., Smith, A.E.: Monomer molecular weight of T-antigen from simian virus 40-infected and -transformed cells. Proc. Natl. Acad. Sci. USA73, 2254–2258 (1976)
Dugaiczyk, A. Boyer, H.W., Goodman, H.M.: Digestion of Eco RI endonucleasegenerated DNA fragments into linear and circular structures. J. Mol. Biol.96, 171–184 (1975)
Falk, L.A., Henle, G., Henle, W., Deinhardt, F., Schudel, A.: Transformation of lymphocytes by Herpesvirus Papio. Int. J. Cancer20, 219–226 (1977)
Fareed, G.E., Davoli, D.: Molecular biology of papovaviruses. Ann. Rev. Biochem.46, 471–522 (1977)
Fiers, W., Contreras, R., Haegeman, G., Rogiers, R., Van de Voorde, A., Van Heuverswyn, H., Van Herreweghe, J., Volckaert, G., Ysebaert, M.: Complete nucleotide sequence of SV 40 DNA. Nature273, 113–120 (1978)
Foley, G.E., Lazarus, H., Farber, S., Uzman, B.G., Adams, R.A.: Studies on human leukemic cells in vitro. In: The proliferation and spread of neoplastic cells. 21st Annual Symposium on Fundamental Cancer Research, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, TX. pp. 65–94. Baltimore. The Williams & Wilkins Co., 1968
Fresen, K.O., zur Hausen, H.: Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV) — genome-negative human lymphoma cells with different EBV strains. Int. J. Cancer17, 161–166 (1976)
Fresen, K.O., Merkt, B., Bornkamm, G.W., zur Hausen, H.: Heterogeneity of Epstein-Barr Virus originating from P3HR-1 cells. I. Studies on EBNA induction. Int. J. Cancer19, 317–323(1977)
Friedman, T., Doolittle, R.F., Walter, G.: Amino acid sequence homology between polyoma and SV 40 tumor antigens deduced from nucleotide sequences. Nature274, 291–292 (1978)
Gardner, S.D., Field, A.M., Coleman, D.V., Hulme, B.: New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet,1971 I, 1253–1257
Gibson, W.: Polyoma virus proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis. Virology62, 391–336 (1974)
Gissmann, L., zur Hausen, H.: Human papilloma viruses: physical mapping and genetic heterogeneity. Proc. Natl. Acad. Sci. USA73, 1310–1313 (1976)
Gissmann, L., Pfister, H., zur Hausen, H.: Human papillomaviruses (HPV): characterization of four different isolates. Virology76, 569–580 (1977)
Harrison, T., Graham, S., Williams, J.: Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology77, 319–329 (1977)
Henle, G., Henle, W.: Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bact.91, 1248–1256 (1966)
Hinuma, Y., Konn, M., Yamaguchi, J. Wudarski, D.J., Blakeslee, J.R., Grace, J.T.: Immunofluorescence and herpes-type particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol.1, 1045–1051 (1976)
Iwakata, S., Grace, J.T.: Cultivation in vitro of myeloblasts from human leukemia. N.Y. State Med. J.64, 2279–2282 (1964)
Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menézes, J., Nilsson, K., Sundström, Ch.: Continuous lymphoid cell lines with characteristics of B cells (bone-marrowderived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc. Natl. Acad. Sci. USA71, 3283–3286 (1974)
Klein, G., Giovanella, B., Westman, A., Stehlin, J.S., Munford, D.: An EBV-Genomenegative cell line established from an American Burkitt lymphoma; receptor characteristics, EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology5, 319–334 (1975)
Lazarus, H., Barell, E.F., Oppenheim, S., Krishnan, A.: Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood. In Vitro9, 303–310 (1974)
Minowada, J., Ohnuma, T., Moore, G.E.: Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst.49, 891–895 (1972)
Padgett, B.L., ZuRhein, G.M., Walker, D.L., Eckrade, R.J.: Cultivation of papovalike virus from human brain with progressive multifocal leukoencephalopathy. Lancet1971 I, 1257–1260
Pulvertaft, R.T.V.: A study of malignant tumors in Nigeria by short term tissue culture. J. Clin. Path.18, 261–273 (1965)
Schachat, F.H., Hogness, D.S.: Repetitive sequences in isolated Thomas circles from Drosophila melanogaster. CSHSQB38, 371–381 (1973)
Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol.98, 503–517 (1975)
Tai, H.R., Smith, C.A., Sharp, P.A., Vinograd, J.: Sequence heterogeneity in closed circular simian virus 40 deoxyribonucleic acid. J. Virol.9, 317–315 (1972)
Waldeck, W., Sauer, G.: New oncogenic papova virus from primate cells. Nature269, 171–173 (1977)
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zur Hausen, H., Gissmann, L. Lymphotropic papovaviruses isolated from African green monkey and human cells. Med Microbiol Immunol 167, 137–153 (1979). https://doi.org/10.1007/BF02121180
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DOI: https://doi.org/10.1007/BF02121180