Abstract
N1 influenza virus neuraminidases (NA) derived from avian, swine and human virus isolates, including the genetically related classic strains A/FPV/Rostock/34, A/Swine/1976/31, A/PR8/34 and A/FM1/47, were analysed serologically by neuraminidase inhibition (NI), inhibition of virus release (IVR) and competitive radio-immunoassays (competitive RIA). Comparing the three tests, competitive RIA appeared to be more reliable than NI and IVR for a quantitative assessment of antigenic relatedness.
Together with evidence presented by others, these studies indicate that the host species contributes to the extent of antigenic variation of NAses. In contrast to NAses of human viruses where antigenic drift occurs readily, NAses of animal influenza viruses, from birds or mammalians, undergo far fewer antigen changes.
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References
Becht H, Malole B (1975) Comparative evaluation of different fixation procedures and different coupling reagents for the demonstration of influenza virus-specific antibodies by indirect hemagglutination test. Med Microbiol Immunol 162:43–53
Blok J, Air GM (1980) Comparative nucleotide sequences at the 3' end of the neuraminidase gene from 11 influenza type A viruses. Virology 107:50–60
Blok J, Air GM (1982) Sequence variation at the 3' end of the neuraminidase gene from 39 influenza type A viruses. Virology 121:211–229
Bolton AE, Hunter WM (1973) The labelling of proteins to high specific radioactivities by conjugation to a125J-containing acylating agent. Biochem J 133:529–539
Bosch FX, Mayer A, Huang RTC (1980) Simple and rapid separation of ortho- and paramyxovirus glycoproteins. Med Microbiol Immunol 168:249–259
Chucholowius HW, Rott R (1972) A new method for purification of myxovirus by zonal centrifugation with two different sucrose density gradients. Proc Soc Exp Biol Med 140:245–247
Davenport FM, Rott R, Schäfer W (1960) Physical and biological properties of influenza virus components obtained after ether treatment. J Exp Med 112:767–782
Desselberger U, Zamecnik P, Palese P (1980) 3'terminal sequences of hemagglutinin and neuraminidase genes of different influenza A viruses. In: Laver WG, Air GM (eds) Structure and variation in influenza virus. Elsevier, New York, pp 169–180
Drzeniek R, Seto JT, Rott R (1966) Characterization of neuraminidases from myxoviruses. Biochim Biophys Acta 128:547–558
Easterday BC (1975) Animal influenza. In: Kilbourne ED (ed) The influenza viruses and influenza. Academic Press, New York, pp 449–482
Fields S, Winter G, Brownlee GG (1981) Structure of the neuraminidase gene in human influenza virus A/PR/8/34. Nature 290:213–217
Hinshaw VS, Bean WJ, Webster RG, Easterday BC (1978) The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses from man and swine. Virology 84:51–62
Hinshaw VS, Bean WJ, Webster RG, Sriram G (1980) Genetic reassortment of influenza viruses in the intestinal tract of ducks. Virology 101:412–419
Hiti AL, Nayak DP (1982) Complete nucleotide sequence of the neuraminidase gene of human influenza virus A/WSN/33. J Virol 41:730–734
Ihle JN, Denny TP, Bolognesi DP (1976) Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice. J Virol 17:727–736
Kendal AP, Kiley MP (1973) Characterization of influenza virus neuraminidases: Peptide changes associated with antigenic divergence between early and late N2 neuraminidases. J Virol 12:1482–1490
Klingeborn B, Rockborn G, Dinter Z (1980) Significant antigenic drift within the influenza equi 2 subtype in Sweden. Vet Rec 106:363–364
Laver WG (1978) Crystallization and peptide maps of neuraminidase “heads” from H2N2 and H3N2 influenza virus strains. Virology 86:78–87
Laver WG, Air GM, Webster RG, Markoff LJ (1982) Amino acid sequence changes in antigenic variants of type A influenza virus N2 neuraminidase. Virology 122:450–460
Markoff L, Lai ChJ (1982) Sequence of the influenza A/Udorn/72 (H3N2) virus neuraminidase gene as determined from cloned full length DNA. Virology 119:288–297
Meier-Ewert H, Gibbs AJ, Dimmock NJ (1970) Studies on antigenic variations of the hemagglutinin and neuraminidase of swine influenza virus isolates. J Gen Virol 6:409–419
Ouchterlony ö (1962) Diffusion-in-gel methods for immunological analysis. Prog Allergy 6:30
Paniker CKJ (1968) Serological relationships between the neuraminidases of influenza viruses. J Gen Virol 2:385–394
Powell DG, Thompson GR, Plowright W, Burrows R, Schild GC (1973) The outbreak of equine influenza in England 1973. Vet Rec 94:284–289
Rott R, Becht H, Orlich M (1974) The significance of influenza virus neuraminidase in immunity. J Gen Virol 22:35–41
Rott R, Becht H, Orlich M (1975) Antigenic relationship between the surface antigens of avian and equine influenza viruses. Med Microbiol Immunol 161:253–261
Schild GC, Oxford JS, Dowdle WR, Coleman M, Pereira MS, Chakraverty P (1973) Antigenic variation in current influenza A viruses: evidence for a high frequency of antigenic “drift” for the Hong Kong virus. Bull WHO 51:1–11
Scholtissek C, Rohde W, Harms E, Rott R (1977) Correlation between base sequence homology of RNA segment 4 and antigenicity of the hemagglutinin of influenza viruses. Virology 79:330–336
Seto JT, Rott R (1966) Functional significance of sialidase during influenza virus multiplication. Virology 30:731–737
Seto JT, Drzeniek R, Rott R (1966) Isolation of a low molecular weight sialidase (neuraminidase) from influenza virus. Biochim Biophys Acta 113:402–404
Shortridge KF, Cherry A, Kendal AP (1979) Further studies of the antigenic properties of H3N2 strains of influenza A isolated from swine in South East Asia. J Gen Virol 44:251–254
Six HR, Kasel JA (1979) Assessment of the antigenic relatedness among H3 hemagglutinins of type A influenza viruses by competition radioimmunoassay. Virology 96:588–595
Webster RG, Laver WG (1975) Antigenic variation of influenza viruses. In: Kilbourne ED (ed) The influenza viruses and influenza. Academic Press, New York pp 209–314
Webster RG, Hinshaw VS, Laver WG (1982) Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies. Virology 117:93–104
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Maywald, F., Bosch, F.X., Orlich, M. et al. Evidence for the contribution of the host species to the extent of antigenic variation of N1 influenza virus neuraminidase. Med Microbiol Immunol 172, 1–11 (1983). https://doi.org/10.1007/BF02123672
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DOI: https://doi.org/10.1007/BF02123672