Abstract
A technique using antibody-coated tubes allows the radioimmunological determination of tetanus antibodies in a simple and rapid way. The procedure is based on the competition for125I-tetanus toxin between the insolubilized antibodies and the antibodies present in the unknown sample. When antibodies and125I labeled tetanus toxin are incubated in the antibody-coated polyethylene vessel the detection limit is about 0.016 IU/ml. When tetanus toxin is preincubated together with its antibody in uncoated vessels before the mixture is transferred to antibody-coated vessels, the sensitivity can be raised about tenfold.
The method is especially useful for screening multiple samples.
Similar content being viewed by others
References
Catt, K., Niall, H. D., Tregear, G. W.: Solid phase radioimmunoassay. Nature (Lond.)213, 825 (1967)
Catt, K., Tregear, G. W.: Solid-phase radioimmunoassay in antibody-coated tubes. Science158, 1570 (1967)
Habermann, E., RÄker, K. O., Zeuner, G.: Solid-phase-Radioimmunassay und Blutspiegel von Staphylokokken-α-Toxin. Naunyn-Schmiedebergs Arch. Pharmak.262, 165 (1969)
Habermann, E., Wiegand, H.: A rapid and simple radioimmunological procedure for measuring low concentrations of tetanus antibodies. Naunyn-Schmiedeberg's Arch. Pharmacol.276, 321 (1973)
Habermann, E., Dimpfel, W.: Distribution of125I-tetanus toxin and125I-toxoid in rats with generalized tetanus, as influenced by antitoxin. Naunyn-Schmiedeberg's Arch. Pharmacol.276, 327 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bernáth, S., Habermann, E. Solid-phase radioimmunoassay in antibody-coated tubes for the quantitative determination of tetanus antibodies. Med Microbiol Immunol 160, 47–51 (1974). https://doi.org/10.1007/BF02124342
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02124342