Summary
Fused-silica capillary columns with 200 μm internal diameter were efficiently packed with both 3 μm and 5 μm spherical C18 bonded-phase particles. Optimum packing conditions were determined and column performance was then critically evaluated in terms of the reduced plate height as a function of both reduced velocity and solute capacity factor. Characteristic values for various column performance indices, such as the Knox separation impedance and the interparticle porosity, were also determined and found to be highly reproducible and similar to those of conventional columns. At optimum velocity, the column contribution to reduced plate height values appeared very close to the theoretical. The columns were found to tolerate samples of up to 5 μg with little loss of efficiency and were used in the high-resolution separation of coal-derived fluids.
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Gluckman, J.C., Hirose, A., McGuffin, V.L. et al. Performance evaluation of slurry-packed capillary columns for liquid chromatography. Chromatographia 17, 303–309 (1983). https://doi.org/10.1007/BF02270662
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DOI: https://doi.org/10.1007/BF02270662