Abstract
Proliferating cells in the gastric mucosal epithelium were successfully enriched by counterflow elutriation in a medium-sized cell fraction. When inoculated on culture plates coated with E-C-L cell attachment matrix, these cells differentiated into mucus-producing cells after reaching confluence. Northern blot analysis did not detect any transcript of the proton pump, histidine decarboxylase, somatostatin, or pepsinogen I, indicating the absence of parietal, ECL, D, and chief cells in the confluent monolayer. These mucus-producing cell monolayers that respond to various growth factors may be a suitable model with which to investigate the function of gastric mucus cells in vitro.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Soll AH, Yamada T, Park J, Thomas LP. Release of somatostatin-like immunoreactivity from canine fundic mucosal cells in primary culture. Am J Physiol 1984;247:G558-G566.
Takahashi M, Ota S, Terano A et al. Hepatocyte, growth factor induces mitogenic reaction to rabbit gastric epithelial cells in primary culture. Biochem Biophys Res Commun 1993;191:528–534.
Ota S, Hata, Y, Terano A, et al. Role of Ca2+ and protein kinase C in regulation of prostaglandin E2 release by cultured rabbit gastric epithelial cells. Dig Dis Sci 1993;38:1426–1436.
Tu Y, Ranta S, Nissinen E, Linden IB. Protection by nitecapone against sodium taurocholate-induced change to cultured gastric cells. Dig Dis Sci 1993;38:701–707.
Schwenk M, Thiedemann KU, Giebel J. Diversity of cell-cell interactions formed by gastric parietal cells in culture: Morphological study of guinea pig cells. J Submicrosc Cytol Pathol 1993;25:333–340.
Soll AH, Toomey M. β-Adrenergic and prostanoid inhibition of canine fundic mucosal mast cells. Am J Physiol 1989;256:G727-G732.
Chiba T, Fisher SK, Park J, et al. Carbamoylcholine and gastrin induce inositol lipid turnover in canine gastric parietal cells. Am J Physiol 1988;255:G99-G105.
Chiba T, Yamada T. Mechanism for muscarinic inhibition of somatostatin release from canine fundic D cells. Metabolism 1990;39:122–124.
Prinz C, Kajimura M, Scott DR, et al. Histamine secretion from rat enterochromaffin like cells. Gastroenterology 1994;105:449–461.
Hattori T. On cell proliferation and differentiation of fundic mucosa of the golden hamster. Cell Tiss Res 1974;148:213–222.
Hattori T, Fujita S. Tritiated thymidine autoradiographic study of cell migration and renewal in the pyloric mucosa of golden hamster. Cell Tiss Res 1976;175:49–57.
Fujimoto S, Hattori T, Kimoto K, et al. Tritiated thymidine autoradiographic study on origin and renewal of gastrin cells in antral area of hamster. Gastroenterology 1980;79:785–791.
Brenna E, Waldum HL. Studies of isolated parietal and enterochromaffin-like cells from the rat. Scand J Gastroenterol 1991;26:1295–1306.
Nakata H, Matsui T, Ito M, et al. Cloning and characterization of gastrin receptor from ECL carcinoid tumor ofMastomya natalensis. Biochem Biophys Res Commun 1992;187:1151–1157.
Yamamoto J, Yasunami K, Ohmori E, et al. cDNA-derived amino acid sequence of L-histidine decarboxylase from mouse mastocytoma P-815 cells. FEBS Lett 1990;276:214–218.
Maeda M, Ishizaki J, Futai M. cDNA cloning and sequence determination of pig gastric H+K+-ATPase. Biochem Biophys Res Commun 1988;157:203–209.
Honda A, Sugimoto Y, Namba T, et al. Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2 subtype. J Biol Chem 1993;268:7759–7762.
Asahara M, Kinoshita Y, Nakata H, et al. Gastrin receptor genes are expressed on gastric parietal and enterochromaffin-like cells ofMastomys natalensis. Dig Dis Sci (in press).
Fujita S, Hattori T. Cell proliferation, differentiation and migration in the gastric mucosa: A study on the background of carcinogenesis. In: Farber F (ed) Pathophysiology of carcinogenesis in the digestive organs. Tokyo: Tokyo University Press/Park Press, 1977;21–36.
Hattori T, Fujita S. Tritiated thymidine autoradiographic study on cellular migration in the gastric gland of the golden hamster. Cell Tiss Res 1976;172:171–184.
Terano A, Ota S, Mach T, et al. Prostaglandin protects against taurocholate-induced damage to rat gastric mucosal cell culture. Gastroenterology 1987;92:669–677.
Okayama N, Itoh M, Joh T, et al. The establishment of a monolayer culture system of guinea pig chief cells and an enzyme immunoassay system for guinea pig pepsinogen. Jpn J Gastroenterol. 1993;90:105–113.
Takao Y, Shimamoto C, Hirata I, Ohshiba. The effect of acid secretagogues on mucin synthesis using primary monolayer culture of the guinea pig gastric mucous cells. Gastroenterol Jpn 1993;28:638–646.
Boland CR, Kraus ER, Scheiman JM, et al. Characterization of mucous cell synthetic functions in a new primary canine gastric mucous cell culture system. Am J Physiol 1990;258:G774-G787.
Lacy ER, Ito S. Rapid epithelial restitution on the rat gastric mucosa after ethanol injury. Lab Invest 1984;51:573–583.
Wright NA, Pike C, Elia G. Induction of a novel epidermal growth factor-secreting cell lineage by mucosal ulceration in human gastrointestinal stem cells. Nature 1990;343:82–85.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kinoshita, Y., Hassan, S., Nakata, H. et al. Establishment of primary epithelial cell culture from elutriated rat gastric mucosal cells. J Gastroenterol 30, 135–141 (1995). https://doi.org/10.1007/BF02348656
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02348656