Abstract
A technique is presented for automatically obtaining the haemoglobin-oxygen equilibrium curve from samples of concentrated haemoglobin solutions or whole blood The amount of oxygen exchanged to the sample across a silicone rubber membrane is calculated from the diffusion equation using a calibrated gas source and the measured pO2 of the sample. With this system it is possible to calculate the entire equilibrium curve from a set of pO2 measurements made as the desaturated sample is raised to an oxygen partial pressure of 150 mm Hg or greater if desired.
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Abbreviations
- P * :
-
Partial pressure of oxygen on gas side of membrane
- P(t) :
-
Measured partial pressure of oxygen in sample as a function of time
- MO 2 :
-
Total quantity of oxygen transferred
- b :
-
Membrane equilibrium time constant
- K :
-
Volume solubility coefficient
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Thibault, L.E., Schuette, W.H. & Lees, D.E. Instrument to determine the haemoglobin-oxygen equilibrium curve based on the calculated diffusion of oxygen across a semi-permeable membrane. Med. Biol. Eng. Comput. 18, 401–405 (1980). https://doi.org/10.1007/BF02443308
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DOI: https://doi.org/10.1007/BF02443308