Summary
The analysis of bile acids in biological matrices, e.g. gall bladder bile and serum, depends on accurate and reproducible sample pre-treatment techniques. The present study compares published and commonly used protocols with a special focus on the analysis of serum bile acids. Since adsorption of bile acids to serum proteins has a dramatic impact on the recovery rate and the reproducibility of the method used, several deproteination procedures were compared. The combination of given protocols made it possible to develop a very sensitive and efficient sample pre-treatment which is well suited for all chromatographic methods for bile acid analysis.
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Abbreviations
- HPLC:
-
high performance liquid chromatography
- HPTLC:
-
high performance thin layer chromatography
- CA:
-
cholate
- LCA:
-
lithocholate
- GCA:
-
glycocholate
- TCA:
-
taurocholate
- GCDCA:
-
glycochenodeoxycholate
- TCDCA:
-
taurochenodeoxycholate
- CDCA:
-
chenodeoxycholate
- GDCA:
-
glycodeoxycholate
- TDCA:
-
taurodeoxycholate
- GLCA:
-
glycolithocholate
- TLCA:
-
taurolithocholate
- GUDCA:
-
glycoursodeoxycholate
- TUDCA:
-
tauroursodeoxycholate
- GHCA:
-
glycohyocholate
- THCA:
-
taurohyocholate
- GHDCA:
-
glycohyodeoxycholate
- THDCA:
-
taurohyodeoxycholate
- α-GMA:
-
α-glycomuricholate
- α-TMA:
-
α-tauromuricholate
- β-GMA:
-
β-glycomuricholate
- β-TMA:
-
β-tauromuricholate
- ω-GMA:
-
ω-glycomuricholate
- ω-TMA:
-
ω-tauromuricholate
- MeOH:
-
methanol
- AcCN:
-
acetonitrile
- CGH:
-
choloylglycine hydrolase
- SPE:
-
solid phase extraction
- BMC:
-
bromomethoxy coumarin
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Dax, C.I., Müllner, S. Convenient and optimized method for sample pre-treatment for the analysis of bile acids in biological matrices. Chromatographia 48, 681–689 (1998). https://doi.org/10.1007/BF02467599
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DOI: https://doi.org/10.1007/BF02467599